BRF1 antibody (AA 501-600)

Catalog No. ABIN1713708

Product Details anti-BRF1 Antibody

Target: BRF1 (BRF1, RNA polymerase III transcription initiation factor subunit (BRF1))

Binding Specificity: AA 501-600

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Cow,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human BRF1/RNA polymerase IIIB 90 kDa subunit

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for BRF1

Target: BRF1 (BRF1, RNA polymerase III transcription initiation factor subunit (BRF1))

Alternative Name: BRF1 (BRF1 Products)

Background:

Synonyms: BRF, CFDS, hBRF, BRF-1, GTF3B, TAF3C, TAF3B2, TF3B90, TAFIII90, TFIIIB90, HEL-S-76p, Transcription factor IIIB 90 kDa subunit, hTFIIIB90, B-related factor 1, TATA box-binding protein-associated factor, RNA polymerase III, subunit 2, BRF1

Background: General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter.

Gene ID: 2972

UniProt: Q92994