BRF1 antibody

Catalog No. ABIN930934

This Mouse Monoclonal antibody specifically detects BRF1 in ICC and DB. It exhibits reactivity toward Human.

Quick Overview for BRF1 antibody (ABIN930934)

Target: BRF1 (BRF1, RNA polymerase III transcription initiation factor subunit (BRF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BRF1 antibody is un-conjugated

Application: Immunocytochemistry (ICC), Dot Blot (DB)

Clone: BRF1G2A8

Product Details anti-BRF1 Antibody

Cross-Reactivity: Human

Cross-Reactivity (Details): Other species not studied.

Purification: Protein G affinity chromatography

Immunogen: BRF1 antibody was raised in mouse using recombinant Brf1 Homolog, Subunit Of Rna Polymerase Iii Transcription Initiation Factor Iiib (S. Cerevisiae) (Brf1)

Isotype: IgG1

Application Details

Application Notes: ICC: 2-100 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: BRF1 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1 % bovine serum albumin (BSA) and 0.05 % sodium azide (NaN3 ).

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for up to one year. We recommend long term storage at -20 °C.

Target Details for BRF1

Target: BRF1 (BRF1, RNA polymerase III transcription initiation factor subunit (BRF1))

Alternative Name: BRF1

Background: This gene encodes one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. The gene product belongs to the TF2B family. Three alternatively spliced variants encoding three different isoforms, that function at different promoters transcribed by RNA polymerase III, have been identified. A transcript encoding a fourth isoform has not yet been completely characterized. Synonyms: Monoclonal BRF1 antibody, Anti-BRF1 antibody, Transcription factor IIIB antibody, BRF antibody, hBRF antibody, GTF3B antibody, TAF3C antibody, TAF3B2 antibody, TF3B90 antibody,TAFIII90 antibody, TFIIIB90 antibody, MGC105048 antibody.