ESAT6 (AA 21-95) antibody

Catalog No. ABIN1715132

Product Details

Target: ESAT6

Binding Specificity: AA 21-95

Reactivity: Bacteria, Mouse, Mycobacteria

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Bacteria, Mouse

Cross-Reactivity (Details): Mycobacterium

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from Mycobacterium tuberculosis ESAT6

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References

Koyama, Ito, Hasegawa, Eriguchi, Inaba, Ushigusa, Sugiura: "Exosomes derived from tumor cells genetically modified to express Mycobacterium tuberculosis antigen: a novel vaccine for cancer therapy." in: Biotechnology letters, Vol. 38, Issue 11, pp. 1857-1866, (2016) (PubMed).

Target Details

Target: ESAT6

Background:

Synonyms: 6 kDa early secretory antigenic target, 6 kDa early secretory antigenic target ESXA, ESAT 6, esaT6, ESXA, ESXA_MYCTU.

Background: Mycobacterium tuberculosis is a slow-growing obligate aerobic bacillus that causes most cases of tuberculosis (TB). It is a small, rod-like microbe that can withstand weak disinfectants and survive in a dry state for weeks, but can only grow within a host organism. M. tuberculosis has a thick waxy cell wall that is responsible for the typical caseous granuloma formation in tuberculosis. ESAT6 is a protein secreted by M. tuberculosis that induces a strong immune response in infected organisms, making it a strong candidate for the development of a vaccine against tuberculosis. ESAT6 may also be useful in the detection of the disease. Deletion of the ESAT6 gene leads to the loss of virulence of M. tuberculosis.