CpxA (N-Term) antibody
Reactivity: Yersinia pseudotuberculosis
WB
Host: Rabbit
Polyclonal
unconjugated
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- Target
- CpxA
- Binding Specificity
- N-Term
- Reactivity
- Yersinia pseudotuberculosis
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Western Blotting (WB)
- Cross-Reactivity (Details)
- Not reactive in: no confirmed exceptions from predicted reactivity are currently known
- Predicted Reactivity
- Yersinia pestis, Yersinia enterocolica and related species
- Purification
- serum
- Immunogen
- Recombinant CpxA (residues 26 to 168) over-expressed as a N-terminal fusion with His(x6)-tag, which was removed prior to immunization. The antigen originates from enteropathogenic Yersinia pseudotuberculosis YPIII, B1JQV1, NCBI annotated locus tag YPK_4133.
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- Application Notes
- 1 : 2000 with standard ECL (WB)
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 50 μL of sterile water.
- Handling Advice
-
Avoid repeated freeze-thaw cycles.
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. - Storage
- -20 °C
- Storage Comment
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- Target
- CpxA
- Background
- CpxA (Conjugative Plasmid Expression) is the 458 amino acid protein, a bacterial inner membrane histidine protein kinase, responsible for sensing misfolded proteins in the periplasm. It is part of CpxA/CpxR two component signal transduction system, where CpxR is the cognate response regulator. In sensing bacterial envelope stress, the Cpx pathway regulates the production of a number of periplasmic folding and trafficking factors to maintain outer membrane integrity. It is also responsible for global control of virulence factor expression. Alternative name: Integral membrane sensor signal transduction histidine kinase.
- Molecular Weight
- expected: 51.8 kDa, appearent: 52-53 kDa
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