Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Specificity
Reacts with human, mouse, and rat Apaf1 (130 kDa).
Purification
Purified
Immunogen
Synthetic peptide, corresponding to amino acids 1158-1177 (C-terminus) of Human Apaf 1. The sequence of the immunogenic peptide differs from that of murine Apaf1 byone amino acid
Working dilution: Optimal dilution should be determined by the end user. The following are guidelines only: IHC(10 - 20 μg/mL) WB(1 - 10 μg/mL) ELISA(1:100 - 1:2000)
Restrictions
For Research Use only
Format
Liquid
Buffer
PBS, pH 7.4
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Dilute in PBS or medium that is identical to that used in the assay system.
Storage
-20 °C
Storage Comment
Stable for at least one year at -20°C. Avoid multiple freeze-thaw cycles.
The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis proteaseactivating factor 1). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3, one of the key proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 is ubiquitously expressed in human tissues.