APAF1 antibody (Apoptotic Peptidase Activating Factor 1) (AA 1158-1177)

Details for Product anti-APAF1 Antibody No. ABIN1731833
Antigen
Epitope
AA 1158-1177, C-Term
41
38
15
13
13
10
5
5
4
4
3
3
2
2
2
2
2
2
2
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
210
134
97
6
3
3
2
1
1
Host
Rabbit
180
25
17
2
Clonality
Polyclonal
Conjugate
This APAF1 antibody is un-conjugated
6
5
5
5
5
3
3
3
3
2
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
157
80
76
52
32
26
22
11
10
7
5
4
3
1
1
Options
Immunogen Synthetic peptide, corresponding to amino acids 1158-1177 (C-terminus) of Human Apaf 1. The sequence of the immunogenic peptide differs from that of murine Apaf1 byone amino acid
Isotype IgG
Specificity Reacts with human, mouse, and rat Apaf1 (130 kDa).
Purification Purified
Antigen
Alternative Name Apaf1 (APAF1 Antibody Abstract)
Background The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis proteaseactivating factor 1). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3, one of the key proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 is ubiquitously expressed in human tissues.

Apoptotic protease-activating factor 1, APAF-1 Gene name: APAF1, KIAA0413, CED4
Gene ID 317
UniProt O14727
Pathways p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity
Application Notes Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
IHC(10 - 20 μg/mL) WB(1 - 10 μg/mL) ELISA(1:100 - 1:2000)
Restrictions For Research Use only
Format Liquid
Buffer PBS, pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Dilute in PBS or medium that is identical to that used in the assay system.
Storage -20 °C
Storage Comment Stable for at least one year at -20°C. Avoid multiple freeze-thaw cycles.
Expiry Date 12 months