Calbindin D28k/D29k antibody
Reactivity: Mouse, Rat
IP, IHC, WB
Host: Mouse
Monoclonal
95C1
unconjugated
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- Target
- Calbindin D28k/D29k
- Reactivity
- Mouse, Rat
- Host
- Mouse
- Clonality
- Monoclonal
- Application
- Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
- Specificity
- Recognizes both isoforms calbindin D28k and D29k / calretinin.
- Purification
- purified IgG. Azide was added before lyophilization.
- Immunogen
- Recombinant full length mouse calretinin.
- Clone
- 95C1
- Isotype
- IgG2b
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- Application Notes
-
WB: 1 : 1000 (AP staining)
ICC: not tested yet
IHC: 1 : 200 up to 1 : 500 - Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- Buffer
- PBS, 0.02% sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
- Storage
- -20 °C
- Storage Comment
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
-
- Target
- Calbindin D28k/D29k
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