SLC6A5 antibody (AA 1-229)

Catalog No. ABIN1742533

Product Details anti-SLC6A5 Antibody

Target: SLC6A5 (Solute Carrier Family 6 (Neurotransmitter Transporter, Glycine), Member 5 (SLC6A5))

Binding Specificity: AA 1-229

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC6A5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Specificity: Specific for glycine transporter 2.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Recombinant protein (aa 1-229 of rat GlYT2)

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
ICC: not tested yet
IHC: 1 : 200

Comment:

WB: Aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References for anti-SLC6A5 antibody (ABIN1742533)

Sunagawa, Shimizu-Okabe, Kim, Kobayashi, Kosaka, Yanagawa, Matsushita, Okabe, Takayama: "Distinct development of the glycinergic terminals in the ventral and dorsal horns of the mouse cervical spinal cord." in: Neuroscience, Vol. 343, pp. 459-471, (2016) (PubMed).

Target Details for SLC6A5

Target: SLC6A5 (Solute Carrier Family 6 (Neurotransmitter Transporter, Glycine), Member 5 (SLC6A5))

Alternative Name: Glycine Transporter 2 (SLC6A5 Products)

Background: Synonyms: GLYT 2, Slc6a5