Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3) (Internal Region) antibody

Details for Product No. ABIN185294
Target
Binding Specificity
Internal Region
Reactivity
Human
Host
Goat
Clonality
Polyclonal
Application
ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
Options
Purpose LXR alpha / LXR beta
Immunogen Peptide with sequence CRLQDKKLPPLLSEI, from the internal region of the protein sequence according to NP_005684.2, NP_009052.3.
Blocking Peptide Blocking peptide for this product available: ABIN369791
Sequence CRLQDKKLPP LLSEI
Isotype IgG
Specificity This antibody is expected to recognise epitopes including aa 429-442 of human LXR alpha protein (NP_005684.2) and aa 443-456 of human LXR beta protein (NP_009052.3).
Predicted Reactivity Human, Mouse, Rat, Dog, Cow
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Target
Alternative Name NR1H3, NR1H2 (NR1H2/3 Antibody Abstract)
Background NR1H3, LXRA, LXR-a, RLD-1, nuclear receptor subfamily 1, group H, member 3, liver X receptor, alpha, NR1H2, NER, UNR, LXR-b, NER-I, RIP15, nuclear receptor subfamily 1, group H, member 2, ubiquitously-expressed nuclear receptor / NR1H2, NER, UNR, LXR-b, NER-I, RIP15, nuclear receptor subfamily 1, group H, member 2, ubiquitously-expressed nuclear receptor, OTTHUMP00000198038, liver X receptor alpha, liver X receptor-alpha, LXRB, LX receptor beta, liver X receptor beta, liver X receptor-beta, nuclear orphan receptor LXR-beta, oxysterols receptor LXR-beta
Gene ID 10062, 7376
NCBI Accession NP_005684, NP_009052, NP_001123573, NP_001238863, NP_001350524
Pathways Nuclear Receptor Transcription Pathway, Cellular Response to Molecule of Bacterial Origin, Hepatitis C
Application Notes

DS WB Results: Approx 55 kDa band observed in Human, Liver and Kidney lysates and in preliminary testing of Human Spleen lysate (calculated MW of 51.1 kDa according to NP_001238863.1). This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on NIH3T3 and Rat for this particular batch.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment

Immunofluorescence: Strong expression of the protein seen in the nuclei of A549 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Images
Image no. 1 for anti-Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3) (Internal Region) antibody (ABIN185294) ABIN185294 (1µg/ml) staining of Human Liver (A) and Kidney (B) lysate (35µg protein i...
Image no. 2 for anti-Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3) (Internal Region) antibody (ABIN185294) ABIN185294 (1µg/ml) staining of human spleen lysate (35µg protein in RIPA buffer). Pr...
Image no. 3 for anti-Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3) (Internal Region) antibody (ABIN185294) ABIN185294 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line),...
Image no. 4 for anti-Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3) (Internal Region) antibody (ABIN185294) ABIN185294 Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabi...