HIST2H2BE antibody (N-Term)

Catalog No. ABIN1881418

Product Details anti-HIST2H2BE Antibody

Target: HIST2H2BE (Histone H2B Type 2-E (HIST2H2BE))

Binding Specificity: AA 12-40, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIST2H2BE antibody is un-conjugated

Application: Western Blotting (WB)

Predicted Reactivity: M

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This HIST2H2BE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-40 amino acids from the N-terminal region of human HIST2H2BE.

Clone: RB41930

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-HIST2H2BE antibody (ABIN1881418)

Kim, Guermah, McGinty, Lee, Tang, Milne, Shilatifard, Muir, Roeder: "RAD6-Mediated transcription-coupled H2B ubiquitylation directly stimulates H3K4 methylation in human cells." in: Cell, Vol. 137, Issue 3, pp. 459-71, (2009) (PubMed).

Pankratova, Manuĭlova, Stepchenko: "[Role of transcription factors binding sites in no-mediated histone H2B promoter repression]." in: Molekuliarnaia biologiia, Vol. 43, Issue 2, pp. 368-73, (2009) (PubMed).

Dai, Yu, Goh, Chng, Tan, Fu, Zheng, Luo: "Histone 2B (H2B) expression is confined to a proper NAD+/NADH redox status." in: The Journal of biological chemistry, Vol. 283, Issue 40, pp. 26894-901, (2008) (PubMed).

Zhao, Lang, Ito, Bonnet, Metzger, Sawatsubashi, Suzuki, Le Guezennec, Stunnenberg, Krasnov, Georgieva, Schüle, Takeyama, Kato, Tora, Devys: "A TFTC/STAGA module mediates histone H2A and H2B deubiquitination, coactivates nuclear receptors, and counteracts heterochromatin silencing." in: Molecular cell, Vol. 29, Issue 1, pp. 92-101, (2008) (PubMed).

Kawasaki, Schiltz, Chiu, Itakura, Taira, Nakatani, Yokoyama: "ATF-2 has intrinsic histone acetyltransferase activity which is modulated by phosphorylation." in: Nature, Vol. 405, Issue 6783, pp. 195-200, (2000) (PubMed).

Target Details for HIST2H2BE

Target: HIST2H2BE (Histone H2B Type 2-E (HIST2H2BE))

Alternative Name: HIST2H2BE (HIST2H2BE Products)

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

Molecular Weight: 13920

NCBI Accession: NP_003519

UniProt: Q16778