Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

LIMK2 antibody (LIM Domain Kinase 2) (pThr505) Primary Antibody

LIMK2 Reactivity: Human, Mouse, Rat IF, IHC (p), WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN196833
$350.90
Plus shipping costs $45.00
50 μL ABIN196833
50 μL ABIN196833
local_shipping Shipping to: United States
Available
  • Target
    LIMK2
    Binding Specificity
    • 12
    • 6
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pThr505
    Reactivity
    • 63
    • 43
    • 40
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 53
    • 3
    • 3
    • 3
    • 1
    Rabbit
    Clonality
    • 61
    • 2
    Polyclonal
    Conjugate
    • 52
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This LIMK2 antibody is un-conjugated
    Application
    • 56
    • 29
    • 26
    • 15
    • 10
    • 6
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Specificity
    This antibody detects endogenous levels of LIMK2 only when phosphorylated at Threonine 505.
    Purification
    Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
    Immunogen
    The antiserum was produced against synthesized phosphopeptide derived from human LIMK2 around the phosphorylation site of threonine 505 (R-Y-TP-V-V).
  • Application Notes
    Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store the antibody (in aliquots) at -20 °C.
  • Target
    LIMK2
    Alternative Name
    LIMK2 (LIMK2 Antibody Abstract)
    Synonyms
    xlimk2, zgc:91990, wu:fa97c09, wu:fk73e11, LIMK2, Limk2b, Link2, LIMK, 9430059K01, A930024P04Rik, C85310, Limk2a, LIM domain kinase 2 L homeolog, LIM domain kinase 2, LIM motif-containing protein kinase 2, limk2.L, limk2, LIMK2, Limk2
    Background
    LIM kinases (LIM kinase 1 and LIM kinase 2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs in their N terminal regulatory domain. LIM kinases are involved in actin cytoskeleton regulation through Rho family GTPases and downstream kinases PAKs and ROCK. PAK1 and ROCK phosphorylate LIM kinase 1 and LIM kinase 2, which increases the activity of the kinases. Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the N terminus of Cofilin.Synonyms: EC=2.7.11.1, LIM domain kinase 2, LIMK-2, LIMK2
    Gene ID
    3985
    NCBI Accession
    NP_005560
    UniProt
    P53671
You are here:
help Support