CD1b antibody

Catalog No. ABIN1981866

Product Details anti-CD1b Antibody

Target: CD1b (CD1B)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD1b antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: The mouse monoclonal antibody SN13 (also known as K5-1B8) recognizes an extracellular epitope of CD1b, a 44 kDa type I glycoprotein associated with beta2-microglobulin. It is expressed on dendritic cells, Langerhans cells, thymocytes, and T acute lymphoblastic leukemia cells.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: A cell membrane antigen preparation that was isolated from normal human thymocytes

Clone: SN13

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze. Do not use after expiration date stamped on vial label.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD1b antibody (ABIN1981866)

Giuliani, Prete, Graziani, Aquino, Balduzzi, Sugita, Brenner, Iona, Fattorini, Orefici, Porcelli, Bonmassar: "Influence of Mycobacterium bovis bacillus Calmette Guérin on in vitro induction of CD1 molecules in human adherent mononuclear cells." in: Infection and immunity, Vol. 69, Issue 12, pp. 7461-70, (2001) (PubMed).

Hayes, Knight: "Group 1 CD1 genes in rabbit." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 1, pp. 403-10, (2001) (PubMed).

Tentori, Graziani, Porcelli, Sugita, Brenner, Madaio, Bonmassar, Giuliani, Aquino: "Rifampin increases cytokine-induced expression of the CD1b molecule in human peripheral blood monocytes." in: Antimicrobial agents and chemotherapy, Vol. 42, Issue 3, pp. 550-4, (1998) (PubMed).

Target Details for CD1b

Target: CD1b (CD1B)

Alternative Name: CD1b (CD1B Products)

Background: CD1b molecule,CD1b (also known as R1) together with CD1a and c, belongs to group 1 of CD1 antigens. These non-classical MHC-like glycoproteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. The trafficking routes of the particular CD1 types differ and correspond to their ability to bind and present different groups of antigens. Besides non-peptide glycolipid antigen presentation to CD1-restricted T cells, CD1b has been implicated in thymocyte development.,R1

Gene ID: 910

UniProt: P29016