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CD16 (CD16) antibody (FITC) Primary Antibody

CD16 Reactivity: Human FACS Host: Mouse Monoclonal 4ACB16 FITC
Catalog No. ABIN2144518
$248.16
Plus shipping costs $45.00
50 tests
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Delivery in 9 to 11 Business Days
  • Target
    CD16
    Reactivity
    • 186
    • 49
    • 16
    • 12
    • 11
    • 10
    • 9
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    Human
    Host
    • 166
    • 38
    • 23
    • 1
    Mouse
    Clonality
    • 198
    • 29
    Monoclonal
    Conjugate
    • 73
    • 25
    • 22
    • 17
    • 13
    • 10
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    FITC
    Application
    • 204
    • 59
    • 50
    • 47
    • 38
    • 30
    • 20
    • 16
    • 10
    • 9
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Characteristics
    4ACB16 reacts with human CD16, a 50-65 kDa Fcg receptor IIIa (FcgRIII), expressed on NK cells, monocytes/macrophages and granulocytes. It is a human NK cell associated antigen. CD16 is a low affinity receptor for IgG which functions in phagocytosis and ADCC, as well as in signal transduction and NK cell activation. The CD16 antibody blocks the binding of soluble immune complexes to granulocytes.This clone is cross reactive with non-human primate.
    Clone
    4ACB16
    Isotype
    IgG1
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Assay Procedure
    • Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
    • Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
    • Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
    • Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
    • [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
    • [Cell fixation: 2 % formaldehyde solution]
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
  • Target
    CD16
    Alternative Name
    CD16 (CD16 Antibody Abstract)
    Synonyms
    Fcgr3, Fcgr3a, CD16, FCGR3, FCGR3@, CD16b, FCG3, FCR-10, FCRIII, FCRIIIb, FcRIII, Fc fragment of IgG, low affinity IIa, receptor, Fc fragment of IgG, low affinity IIIa, receptor (CD16a), Fc fragment of IgG receptor IIIb, Fc fragment of IgG receptor IIIa, Fc receptor, IgG, low affinity III, Fc-gamma RIII, Fcgr2a, FCGR3A, FCGR3B, Fcgr3, FCGR3
    Pathways
    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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