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C5a Receptor antibody

C5AR Reactivity: Rat IHC (fro), FACS, WB Host: Mouse Monoclonal R63 unconjugated
Pubmed (4 references)
Catalog No. ABIN2192106
$797.69
Plus shipping costs $45.00
100 μg
Shipping to: United States
Delivery in 6 to 8 Business Days
  • Target See all C5a Receptor (C5AR) products
    C5a Receptor (C5AR)
    Reactivity
    • 2
    • 2
    Rat
    Host
    • 2
    • 2
    Mouse
    Clonality
    • 4
    Monoclonal
    Application
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Western Blotting (WB)
    Sterility
    0.2 μm filtered
    Clone
    R63
  • Application Notes
    For immunohistochemistry, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
    Restrictions
    For Research Use only
  • Buffer
    PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
  • Crane, Baiquni, Sullivan, Lee, Sah, Taylor, Noakes, Woodruff: "The C5a anaphylatoxin receptor CD88 is expressed in presynaptic terminals of hippocampal mossy fibres." in: Journal of neuroinflammation, Vol. 6, pp. 34, (2009) (PubMed).

    Woodruff, Costantini, Crane, Atkin, Monk, Taylor, Noakes: "The complement factor C5a contributes to pathology in a rat model of amyotrophic lateral sclerosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 12, pp. 8727-34, (2008) (PubMed).

    Griffin, Costigan, Brenner, Ma, Scholz, Moss, Allchorne, Stahl, Woolf: "Complement induction in spinal cord microglia results in anaphylatoxin C5a-mediated pain hypersensitivity." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 32, pp. 8699-708, (2007) (PubMed).

    Schieferdecker, Schlaf, Koleva, Götze, Jungermann: "Induction of functional anaphylatoxin C5a receptors on hepatocytes by in vivo treatment of rats with IL-6." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 10, pp. 5453-8, (2000) (PubMed).

  • Target
    C5a Receptor (C5AR)
    Alternative Name
    c5a Receptor (C5AR Products)
    Background
    The monoclonal antibody R63 reacts with the rat receptor for C5a (CD88). The rat anaphylatoxin C5a is a 77-amino acid glycopolypeptide which is generated by proteolytic cleavage of the complement factor C5 in the course of complement activation. A variety of biological effects evoked by C5a has been demonstrated, rendering this molecule an important mediator of inflammation, with granulocytes and macrophages as the main target cells. All cellular responses to C5a are specifically mediated by interactions with the membrane bound C5a receptor, a seven transmembrane GTP-binding-protein- coupled receptor that belongs to the rhodopsin supergene family. The 45 kDa C5a receptor is expressed by myeloid cells including Kupfer cells. Using immunohistology there is no evidence for non-myeloid expression in healthy control rats. Under inflammatory situations however the C5aR was found to be upregulated in various organs and tissues including the liver. Aliases C5aR, CD88, C5a anaphylatoxin chemotactic receptor, C5a anaphylatoxin receptor, C5a ligand, C5a- R, complement component 5a receptor 1 Immunogen RBH-2H3 cells transfected with rat C5aR gene
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