Ethenoadenosine antibody

Catalog No. ABIN2192142

Product Details

Target: Ethenoadenosine

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunoprecipitation (IP), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoassay (IA)

Sterility: 0.2 μm filtered

Clone: 1G4

Application Details

Application Notes: For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

Target Details

Target: Ethenoadenosine

Target Type: Chemical

Background: The reaction of chloroacetaldehyde (CAA) with DNA results in the formation of ethenoadenosine also known as 1,N6-ethenodeoxy-adenosine or etheno-A. CAA is formed through metabolization of for example vinyl chloride, a well established carcinogen. In RNA, after in vitro activation, etheno-A and etheno-C are the principle products of RNA damage. Etheno derivate formation may be highest in single-stranded DNA- regions. The monoclonal antibody 1G4 reacts with both the ribose and deoxyribose form of the adduct. The antibody is not cross reactive with non-modified DNA or the normal nucleotides.