Secretory Leucocyte Protease Inhibitor antibody

Catalog No. ABIN2192189

Product Details

Target: Secretory Leucocyte Protease Inhibitor

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Application: Immunoassay (IA), Immunoprecipitation (IP), Western Blotting (WB)

Sterility: 0.2 μm filtered

Application Details

Application Notes: For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References

Kramps, van Twisk, Klasen, Dijkman: "Interactions among stimulated human polymorphonuclear leucocytes, released elastase and bronchial antileucoprotease." in: Clinical science (London, England : 1979), Vol. 75, Issue 1, pp. 53-62, (1988) (PubMed).

Target Details

Target: Secretory Leucocyte Protease Inhibitor

Background: The antibody reacts with secretory leukocyte proteinase inhibitor (SLPI, also known as antileukoprotease (ALP)). SLPI is a 11.7 kDa cationic inhibitor of neutrophil elastase and to a lesser extent of cathepsin G. It is locally produced by epithelial cells in the lung, skin and other organs, by Polymorphonuclear leukocytes (PMN) and (in mice) by macrophages. In addition to its proteinase inhibitory properties that may serve to protect against proteolytic injury, it was recently shown that SLPI also displays several other functions such as antimicrobial and anti-inflammatory activities. These appear to be independent of its ability to inhibit PMN serine proteinases. SLPI has also been demonstrated to display antibacterial and antifungal activity at concentrations in which SLPI is present in mucosal secretions including those of the lung. Another possible role for SLPI is inhibition of protein-disulphide isomerase that is considered essential for invasion of a cell by the Human Immunodeficiency Virus (HIV).