It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Restrictions
For Research Use only
Buffer
PBS, containing 0.1 % bovine serum albumin
Storage
4 °C
Leeuwenberg, Dentener, Buurman: "Lipopolysaccharide LPS-mediated soluble TNF receptor release and TNF receptor expression by monocytes. Role of CD14, LPS binding protein, and bactericidal/permeability-increasing protein." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 10, pp. 5070-6, (1994) (PubMed).
Target
TNFRSF1B
(Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))
The antibody MR2-1 reacts with the extra-cellular part of the TNF-RII. It also reacts with the soluble receptor. TNF-RII is present on most cell types and is considered to play a prominent role in cell stimulation by TNF-alpha. TNF-RII molecule is shown to be responsible for stimulation of activated T-lymphocytes by TNF-alpha. The antibody cross reacts with rhesus and cynomolgus natural TNF-RII. Mouse IgG1