SLC12A4 antibody (Internal Region)

Catalog No. ABIN238576

Product Details anti-SLC12A4 Antibody

Target: SLC12A4 (Solute Carrier Family 12 (Potassium-Chloride Transporter) Member 4 (SLC12A4))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SLC12A4 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Purpose: KCC1 / SLC12A4

Sequence: DPSHAPDNFR E

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-DPSHAPDNFRE, from the internal region of the protein sequence according to NP_005063.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Placenta. Recommended concentration: 5 μg/mL.

Western Blot: Approx 150 kDa band observed in Human Heart lysates (calculated MW of 121 kDa according to NP_005063.1). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-SLC12A4 antibody (ABIN238576)

Wei, Akerman, Newey, Pan, Clinch, Jacob, Shen, Wilkins, Ellory: "The potassium-chloride cotransporter 2 promotes cervical cancer cell migration and invasion by an ion transport-independent mechanism." in: The Journal of physiology, Vol. 589, Issue Pt 22, pp. 5349-59, (2011) (PubMed).

Target Details for SLC12A4

Target: SLC12A4 (Solute Carrier Family 12 (Potassium-Chloride Transporter) Member 4 (SLC12A4))

Alternative Name: SLC12A4 (SLC12A4 Products)

Background: SLC12A4, KCC1, solute carrier family 12 (potassium/chloride transporters), member 4, FLJ40489, potassium transport protein, potassium/chloride cotransporter 1

Gene ID: 6560, 20498, 29501

NCBI Accession: NP_005063