HCV Core Protein antibody (AA 13-124, AA 369-704) (FITC)

Catalog No. ABIN2451998

Product Details anti-HCV Core Protein Antibody (FITC)

Target: HCV Core Protein (HCV C) (Hepatitis C Virus Core Protein (HCV C))

Binding Specificity: AA 13-124, AA 369-704

Reactivity: Hepatitis C Virus (HCV)

Host: Mouse

Clonality: Monoclonal

Conjugate: This HCV Core Protein antibody is conjugated to FITC

Application: Immunofluorescence (IF), Immunohistochemistry (IHC)

Specificity: Specific to human HCV core antigen of genotype 1b.

Cross-Reactivity (Details): Not tested in other genotypes.

Sterility: Sterile filtered

Immunogen: A part of the core region (nucleotides 369-704, aa 13-124) of HCV genotype 1b expressed in E. coli

Clone: H6-29

Isotype: IgG2a kappa

Application Details

Application Notes: 1. Immunofluorescence staining
2. Immunohistochemistry

Comment:

Conjugate: FITC conjugated, [FITC] / [IgG] = 6.7

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.6 mg/mL

Buffer: PBS with 50 % Glycerol

Preservative: Azide free

Storage: -20 °C

References for anti-HCV Core Protein antibody (FITC) (ABIN2451998)

Brass, Moradpour, Blum: "Molecular virology of hepatitis C virus (HCV): 2006 update." in: International journal of medical sciences, Vol. 3, Issue 2, pp. 29-34, (2006) (PubMed).

Manabe, Fuke, Tanishita, Kaji, Gomi, Yoshida, Mori, Takamizawa, Yosida, Okayama: "Production of nonstructural proteins of hepatitis C virus requires a putative viral protease encoded by NS3." in: Virology, Vol. 198, Issue 2, pp. 636-44, (1994) (PubMed).

Hiramatsu, Hayashi, Haruna, Kasahara, Fusamoto, Mori, Fuke, Okayama, Kamada: "Immunohistochemical detection of hepatitis C virus-infected hepatocytes in chronic liver disease with monoclonal antibodies to core, envelope and NS3 regions of the hepatitis C virus genome." in: Hepatology (Baltimore, Md.), Vol. 16, Issue 2, pp. 306-11, (1992) (PubMed).

Takamizawa, Mori, Fuke, Manabe, Murakami, Fujita, Onishi, Andoh, Yoshida, Okayama: "Structure and organization of the hepatitis C virus genome isolated from human carriers." in: Journal of virology, Vol. 65, Issue 3, pp. 1105-13, (1991) (PubMed).

Kato, Hijikata, Ootsuyama, Nakagawa, Ohkoshi, Sugimura, Shimotohno: "Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 24, pp. 9524-8, (1991) (PubMed).

Target Details for HCV Core Protein

Target: HCV Core Protein (HCV C) (Hepatitis C Virus Core Protein (HCV C))

Alternative Name: HCV Core Protein (HCV C Products)

Target Type: Viral Protein

Background: Hepatitis C virus (HCV) is a small (55-65 nm in size), enveloped, positive sense single-stranded RNA virus in the family Flaviviridae and the principal cause of parenteral non-A, non-B hepatitis. The virus genome consists of a single open reading frame of approximately 9.4 kb which encodes a single polyprotein of about 3,010 amino acids. The polyprotein is processed by host cell and viral proteases into four structural proteins (core, envelope1 and 2, and p7) and six non-structural proteins (NS2, 3, 4a, 4b, 5a, and 5b) necessary for viral replication. HCV core protein (191 aa) is not only a component of nucleocapsid but also has multiple functions and is thought to be a pathogenic factor for hepatitis. It also participates in some cellular processes, including transcriptional regulation and cellular transduction. HCV core antigen is used as diagnostic marker for HCV infection.