Sup35 (AA 494-507), (PSI+ Prion Phenotype) antibody

Catalog No. ABIN2452140

Product Details

Target: Sup35

Binding Specificity: AA 494-507, PSI+ Prion Phenotype

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Cross-Reactivity (Details): Not tested in other species.

Characteristics: Rabbit polyclonal antibody,purified IgG fraction

Purification: Purified

Immunogen: Synthetic peptide corresponding to a.a. 494-507 of Sup35

Isotype: IgG

Application Details

Application Notes: 1) Western blotting: 1,000~2,000 fold dilution.
Not tested for other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 4 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: -20 C (For long term storage: -70 C)

References

Kimura, Koitabashi, Kakizuka, Fujita: "The role of pre-existing aggregates in Hsp104-dependent polyglutamine aggregate formation and epigenetic change of yeast prions." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 9, Issue 8, pp. 685-96, (2004) (PubMed).

Target Details

Target: Sup35

Background: Background: Sup35 protein of S. cerevisiae is the translation termination factor eRF3. The altered conformation of this protein generates the [PSI+] prion phenotype . In this state, a dominant cytoplasmically inherited protein aggregates are formed which sequester the normal function of Sup35 thereby nonsense suppressor phenotype is created . The molecular chaperon Hsp104 is necessary for the formation and maintenance of the aggregates .

UniProt: P05453