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Sup35 (AA 494-507), (PSI+ Prion Phenotype) antibody

Reactivity: Saccharomyces cerevisiae WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN2452140
  • Target
    Sup35
    Binding Specificity
    AA 494-507, PSI+ Prion Phenotype
    Reactivity
    Saccharomyces cerevisiae
    Host
    Rabbit
    Clonality
    Polyclonal
    Application
    Western Blotting (WB)
    Cross-Reactivity (Details)
    Not tested in other species.
    Characteristics
    Rabbit polyclonal antibody,purified IgG fraction
    Purification
    Purified
    Immunogen
    Synthetic peptide corresponding to a.a. 494-507 of Sup35
    Isotype
    IgG
  • Application Notes
    1) Western blotting: 1,000~2,000 fold dilution.
    Not tested for other applications.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    4 mg/mL
    Buffer
    PBS, 50 % glycerol, 0.09 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C/-80 °C
    Storage Comment
    -20 C (For long term storage: -70 C)
  • Kimura, Koitabashi, Kakizuka, Fujita: "The role of pre-existing aggregates in Hsp104-dependent polyglutamine aggregate formation and epigenetic change of yeast prions." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 9, Issue 8, pp. 685-96, (2004) (PubMed).

  • Target
    Sup35
    Background
    Background: Sup35 protein of S. cerevisiae is the translation termination factor eRF3. The altered conformation of this protein generates the [PSI+] prion phenotype . In this state, a dominant cytoplasmically inherited protein aggregates are formed which sequester the normal function of Sup35 thereby nonsense suppressor phenotype is created . The molecular chaperon Hsp104 is necessary for the formation and maintenance of the aggregates .
    UniProt
    P05453
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