This antibody reacts with human J chain as tested by ELISA using purified human Ig fragments as the solid phase target antigen.The antibody does not detect J chain in intact IgM in ELISA assays due to steric hindrance. In Western blotting techniques This antibody detects J chain in IgM only in reducing conditions.
IGJ
Reactivity: Human
WB
Host: Mouse
Polyclonal
unconjugated
Application Notes
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Arnold, Wormald, Suter, Radcliffe, Harvey, Dwek, Rudd, Sim: "Human serum IgM glycosylation: identification of glycoforms that can bind to mannan-binding lectin." in: The Journal of biological chemistry, Vol. 280, Issue 32, pp. 29080-7, (2005) (PubMed).
Target
IGJ
(Immunoglobulin J Polypeptide, Linker Protein For Immunoglobulin alpha and mu Polypeptides (IGJ))