CD74 antibody (HLA-DR-gamma) (Atto 488)

Details for Product anti-CD74 Antibody No. ABIN2481699
Antigen
  • DHLAG
  • HLADG
  • II
  • Ia-GAMMA
  • CLIP
  • Ii
  • INVG34
  • Iclp-1
  • iclp1
  • wu:fb11f09
  • wu:fb12a01
  • wu:fb13d06
  • wu:fk94e07
  • CD74 molecule
  • CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)
  • CD74 molecule, major histocompatibility complex, class II invariant chain a
  • CD74
  • Cd74
  • cd74a
Reactivity
Human, Mouse (Murine)
481
102
31
27
Host
Mouse
408
104
2
2
Clonality (Clone)
Monoclonal ()
Conjugate
This CD74 antibody is conjugated to Atto 488
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30
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14
12
7
7
7
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5
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4
4
4
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4
4
4
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
309
297
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100
76
63
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40
14
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1
Options
Immunogen Human CD74 invariant chain synthetic peptide
Clone PIN-1
Isotype IgG1
Specificity Detects ~33-35 kDa protein doublet corresponding to the molecular mass of the p33 and p35 forms of human CD74.
Purification Protein G Purified
Plasmids, Primers & others Plasmids, Primers & others CD74 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name CD74 (CD74 Antibody Abstract)
Background CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids, followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain (1). The CD74 chain is thought to function mainly as an MHC class II chaperone, which promotes ER exit of MHC class II molecules, directs them to endocytic compartments, prevents peptide binding in the ER, and contributes to peptide editing in the MHC class II compartment. Class II MHC and Ii expression was believed to be restricted to classical antigen-presenting cells (APC), however, during inflammation, other cell types, including mucosal epithelial cells, have also been reported to express class II MHC molecules (2). Experiments that investigate cell-surface CD74 are complicated by the fact that CD74 remains on the cell surface for a very short time. The surface half-life of CD74 was calculated to be fewer than 10 minutes (3). CD74 however has also recently been shown to have a role as an accessory-signaling molecule because of its high-affinity binding to the pro-inflammatory cytokine, macrophage migration-inhibitory factor (MIF) (3). The restricted expression of CD74 by normal tissues and its very rapid internalization make CD74 an attractive therapeutic target for both cancer and immunologic diseases (4).
Gene ID 972
NCBI Accession NP_001020329
UniProt P04233
Pathways Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints
Application Notes
  • WB (1:1000)
  • IHC (1:100)
  • ICC/IF (1:50)
  • optimal dilutions for assays should be determined by the user.
Comment

1 μg/ml of ABIN2481699 was sufficient for detection of CD74 in 20 μg of PALA cell lysates by colorimetric immunolot analysis using goat anti-mouse IgG: AP as the secondary antibody.

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Image no. 1 for anti-HLA-DR-gamma (CD74) antibody (Atto 488) (ABIN2481699) Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CD74 Monoclonal Anti...
Image no. 2 for anti-HLA-DR-gamma (CD74) antibody (Atto 488) (ABIN2481699) Immunohistochemistry analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone PIN 1....
Image no. 3 for anti-HLA-DR-gamma (CD74) antibody (Atto 488) (ABIN2481699) Western Blot analysis of Human N87 cell lysates showing detection of CD74 protein usi...
Image no. 4 for anti-HLA-DR-gamma (CD74) antibody (Atto 488) (ABIN2481699) Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CD74 Monoclonal Anti...
Background publications Denzin, Robbins, Carboy-Newcomb, Cresswell: "Assembly and intracellular transport of HLA-DM and correction of the class II antigen-processing defect in T2 cells." in: Immunity, Vol. 1, Issue 7, pp. 595-606, 1995 (PubMed).

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