Alginate antibody (Biotin)

Catalog No. ABIN2484867

Product Details

Target: Alginate

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: Biotin

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: Binds selectively to a BSA-conjugated alginate, but not to unconjugated BSA.

Purification: Protein G Purified

Immunogen: Sodium Alginate conjugated to KLH

Clone: 4B10-1C5

Isotype: IgG1 kappa

Application Details

Application Notes:

• WB (1:500)
• IHC (1:200)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2484867 was sufficient for detection of 150 ng of alginate-conjugated BSA by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details

Target: Alginate

Target Type: Chemical

Background: Sodium alginate is used in biological experiments for the immobilization of cells and encapsulation due to its biocompatibility and simple gelation with divalent cations such as Ca2+. Studies suggest that preparation of alginate microspheres will sustain protein delivery within tissue scaffolds (1), as well as in many other cell types (2).