AtpH antibody
Reactivity: Arabidopsis thaliana, Nicotiana tabacum, Spinach, Thermosynechococcus elongatus
WB
Host: Rabbit
Polyclonal
unconjugated
1 image
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- Target
- AtpH
- Reactivity
- Arabidopsis thaliana, Nicotiana tabacum, Spinach, Thermosynechococcus elongatus
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
- Application
- Western Blotting (WB)
- Specificity
- The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthasesubcomplex, as well as the chloroplastic CF1 ATP synthase and most knownbacterial F-type ATP synthases.
- Cross-Reactivity (Details)
- Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
- Predicted Reactivity
- dicots including Glycine max, Pisum sativum, Vitis vinifera, monocolts indlucing Oryza sativa, Zea mays, trees: Populus alba, Pinus thunbergii, moss: Physcomitrella patens, green algae
- Characteristics
- Expected / apparent Molecular Weight of the Antigene: 8 kDa (for Arabidopsis thaliana)
- Purification
- serum
- Immunogen
- purified c subunit from Spinacia oleracea P69447
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anti-AtpH antibody
Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii WB Host: Rabbit Polyclonal unconjugated
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- Application Notes
- 1: 1000 - 1: 10 000 with standard ECL (WB)
- Comment
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please note that increased incubation at 95°C (20-30 min) prior to loading is recommended to break the multimeric c-mer structure, detection of partial ring structures (e.g. 5 or 6 subunits) may occur
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 200 μL of sterile water
- Handling Advice
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - Storage
- -20 °C
- Storage Comment
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- Target
- AtpH
- Background
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AGI Code: ATCG00140
F-type ATPase (ATP synthase) is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. Multiple copies of the c subunit build up the ring structure (in spinach a 14-mer of ~112 kDa) of the membrane bound Fo-part of the enzyme. - Molecular Weight
- 8 kDa (for Arabidopsis thaliana)
- UniProt
- P69447
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