Chloroplastic Degp-Type Serine Protease 1 (Deg1) antibody

Catalog No. ABIN249352

Product Details

Target: Chloroplastic Degp-Type Serine Protease 1 (Deg1)

Reactivity: Arabidopsis thaliana, Pisum sativum, Zea mays

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Predicted Reactivity: dicots including: Ricinus communis

Characteristics: Expected / apparent Molecular Weight of the Antigene: 46.6 / 36 kDa

Purification: affinity purified

Immunogen: Recombinant Deg1 of Arabidopsis thaliana O22609, At3g27925, overexpressed in E.coli

Isotype: IgG

Application Details

Application Notes: 1: 500 on 5 μg thylakoid fraction (WB)

Comment:

unspecific reaction at ca. 30 kDa and ca. 85 kDa (checked by by mass spec, and confirmed not to be neither Deg1 nor any other protease. The band at the right MW does contain Deg1 by our MS analysis

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water.

Buffer: PBS pH 7.4

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details

Target: Chloroplastic Degp-Type Serine Protease 1 (Deg1)

Background: AGI Code: At3g27925
Deg1 belongs to S1 chymotrypsin protease family and contains 16 protein members in Arabidopsis thaliana. Deg1 is localized to chloroplast.

Molecular Weight: expected: 46.6 kDa, apparent: 36 kDa

UniProt: O22609