Myeloid/lymphoid Or Mixed-Lineage Leukemia (Trithorax Homolog, Drosophila) (MLL) (AA 2829-2883) antibody

Details for Product No. ABIN2668494
Antigen
Epitope
AA 2829-2883
10
9
7
7
4
2
2
2
2
1
1
1
Reactivity
Human, Mouse (Murine)
57
16
11
Host
Rabbit
44
13
Clonality
Polyclonal
Conjugate
Un-conjugated
3
2
2
2
2
2
1
1
1
1
1
1
Application
ChIP DNA-Sequencing (ChIP-seq), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
33
31
28
11
5
3
2
2
1
1
1
Options
Immunogen This MLL / HRX antibody was raised against a recombinant protein corresponding to amino acids 2829-2883 of human MLL.
Isotype IgG
Purification Protein A Chromatography
Antigen
Alternative Name MLL / HRX (MLL Antibody Abstract)
Background MLL (myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila)), also known as Histone-lysine N-methyltransferase HRX, is a histone methyltransferase that plays an essential role in early development and hematopoiesis. Catalytic subunit of the MLL1 /MLL complex, a multiprotein complex that mediates both methylation of 'Lys-4' of histone H3 (H3K4me) complex and acetylation of 'Lys-16' of histone H4 (H4K16ac). In the MLL1/MLL complex, it specifically mediates H3K4me, a specific tag for epigenetic transcriptional activation. Has weak methyltransferase activity by itself, and requires other component of the MLL1/MLL complex to obtain full methyltransferase activity. Has no activity toward histone H3 phosphorylated on 'Thr-3', less activity toward H3 dimethylated on 'Arg-8' or 'Lys-9', while it has higher activity toward H3 acetylated on 'Lys-9'. Required for transcriptional activation of HOXA9. Promotes PPP1R15A-induced apoptosis.
Molecular Weight 180 kDa
Gene ID 4297
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 μg/μL
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage -20 °C
Storage Comment Antibodies in solution can be stored at -20 °C for 2 years.
Expiry Date 6 months
Supplier Images
Image no. 1 for anti-Myeloid/lymphoid Or Mixed-Lineage Leukemia (Trithorax Homolog, Drosophila) (MLL) (AA 2829-2883) antibody (ABIN2668494) MLL / HRX antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® Hi...
Image no. 2 for anti-Myeloid/lymphoid Or Mixed-Lineage Leukemia (Trithorax Homolog, Drosophila) (MLL) (AA 2829-2883) antibody (ABIN2668494) MLL / HRX antibody (pAb) tested by Immunofluorescence. Formaldehyde fixed HeLa cells ...
Product cited in: Kühn, Löscher, Marschalek: "The IRX1/HOXA connection: insights into a novel t(4;11)- specific cancer mechanism." in: Oncotarget, Vol. 7, Issue 23, pp. 35341-52, 2016 (PubMed).

Kang, Piao, Ryu, Kang, Chang, Keum, Hyun: "Interaction of DNA demethylase and histone methyltransferase upregulates Nrf2 in 5-fluorouracil-resistant colon cancer cells." in: Oncotarget, Vol. 7, Issue 26, pp. 40594-40620, 2016 (PubMed).

Ahmad, Katryniok, Scholz, Merkens, Löscher, Marschalek, Steinhilber: "Inhibition of class I HDACs abrogates the dominant effect of MLL-AF4 by activation of wild-type MLL." in: Oncogenesis, Vol. 3, pp. e127, 2014 (PubMed).

Pradeepa, Grimes, Taylor, Sutherland, Bickmore: "Psip1/Ledgf p75 restrains Hox gene expression by recruiting both trithorax and polycomb group proteins." in: Nucleic acids research, Vol. 42, Issue 14, pp. 9021-32, 2014 (PubMed).

Wilkinson, Ballabio, Geng, North, Tapia, Kerry, Biswas, Roeder, Allis, Melnick, de Bruijn, Milne: "RUNX1 is a key target in t(4;11) leukemias that contributes to gene activation through an AF4-MLL complex interaction." in: Cell reports, Vol. 3, Issue 1, pp. 116-27, 2013 (PubMed).