ITGA5 antibody (Integrin, alpha 5 (Fibronectin Receptor, alpha Polypeptide)) (Biotin)

Details for Product anti-ITGA5 Antibody No. ABIN2689318
Antigen
  • ITGA5
  • Cd49e
  • Fnra
  • VLA5
  • CD49e
  • FNRA
  • VLA5A
  • Itga5_retired
  • integrin subunit alpha 5
  • integrin alpha 5 (fibronectin receptor alpha)
  • ITGA5
  • Itga5
Alternatives
anti-Human ITGA5 antibody for Flow Cytometry
Reactivity
Mouse (Murine)
197
150
56
5
4
2
2
1
1
1
Host
Rat
148
57
53
10
2
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This ITGA5 antibody is conjugated to Biotin
28
22
13
6
4
4
4
4
3
3
3
3
1
1
1
1
1
Application
Flow Cytometry (FACS)
137
134
88
78
31
29
18
14
13
7
7
5
3
2
2
1
1
Options
Brand BD Pharmingen™
Immunogen Mouse mast cell line MC/9
Clone 5H10
Isotype IgG2a, kappa
Characteristics The 5H10-27 (MFR5) antibody reacts with the α5 chain of the integrin α5β1 fibronectin receptor (CD49e/CD29, VLA-5) on thymocytes, activated T lymphocytes, mast cells, and a variety of mouse cell lines, but not splenocytes. Soluble 5H10-27 (MFR5) antibody has been reported to inhibit VLA-5-mediated functions in vitro. In addition, immobilized mAb 5H10-27 (MFR5) has been demonstrated to costimulate the proliferative response of CD8+ T cells to plate-bound anti-CD3e antibody. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Biotin Rat Anti-Mouse CD49e - Biotin - Clone 5H10-27 (MFR5) - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Plasmids, Primers & others Plasmids, Primers & others ITGA5 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name CD49e (ITGA5 Antibody Abstract)
Background Synonyms: Integrin α5 chain
Pathways Integrin Complex
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Storage 4 °C
Storage Comment Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Product cited in: Cukierman, Pankov, Stevens, Yamada: "Taking cell-matrix adhesions to the third dimension." in: Science (New York, N.Y.), Vol. 294, Issue 5547, pp. 1708-12, 2001 (PubMed).

Rich, Van Nood, Lee: "Role of alpha 5 beta 1 integrin in TGF-beta 1-costimulated CD8+ T cell growth and apoptosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 7, pp. 2916-23, 1996 (PubMed).

Ruppert, Aigner, Hubbe, Yagita, Altevogt: "The L1 adhesion molecule is a cellular ligand for VLA-5." in: The Journal of cell biology, Vol. 131, Issue 6 Pt 2, pp. 1881-91, 1996 (PubMed).

Halvorson, Coligan: "Enhancement of VLA integrin receptor function on thymocytes by cAMP is dependent on the maturation stage of the thymocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 10, pp. 4567-74, 1995 (PubMed).

Hardy, Minguell: "Modulation of the adhesion of hemopoietic progenitor cells to the RGD site of fibronectin by interleukin 3." in: Journal of cellular physiology, Vol. 164, Issue 2, pp. 315-23, 1995 (PubMed).

Kinashi, Springer: "Adhesion molecules in hematopoietic cells." in: Blood cells, Vol. 20, Issue 1, pp. 25-44, 1995 (PubMed).

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