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Chromogranin A antibody

CHGA Reactivity: Human IF, WB, ICS, BI Host: Mouse Monoclonal S21 unconjugated
Catalog No. ABIN2689472
  • Target See all Chromogranin A (CHGA) Antibodies
    Chromogranin A (CHGA)
    Reactivity
    • 199
    • 73
    • 66
    • 28
    • 28
    • 15
    • 6
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    Human
    Host
    • 106
    • 98
    • 5
    • 1
    Mouse
    Clonality
    • 125
    • 84
    Monoclonal
    Conjugate
    • 93
    • 18
    • 11
    • 10
    • 10
    • 10
    • 10
    • 10
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Chromogranin A antibody is un-conjugated
    Application
    • 113
    • 107
    • 70
    • 66
    • 60
    • 50
    • 32
    • 13
    • 13
    • 11
    • 10
    • 4
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    Immunofluorescence (IF), Western Blotting (WB), Intracellular Staining (ICS), BioImaging (BI)
    Brand
    BD Pharmingen™
    Characteristics
    Chromogranin A (CGA) is a member of the granin family of regulated secretory proteins that are found in secretory granules in endocrine and neuroendocrine cells and released in response to extracellular stimulation. Intracellularly, granins are important for targeting peptide hormones and neurotransmitters by their ability to aggregate in the low pH , high calcium environment of the trans-Golgi network. Extracellularly, peptides formed from proteolytic processing of granins regulate hormone secretion. CGA is a prohormone that can be cleaved into several biologically active peptides, such as pancreastatin, β-granin, vasostatin, catestatin, and parastatin. β-granin is an N-terminal fragment of CGA, while pancreastatin and catestatin are processed from the central region of CGA. Cells of the adrenal medulla, anterior pituitary, cerebral cortex as well as beta cells of the pancreas and a variety of tumor cell lines express CGA. The expression of CGA can be used to monitor the pancreatic differentiation of pluripotent stem cells. Flow cytometric analysis of Chromogranin A expression in human neuroblastoma cells. SH-SY5Y neuroblastoma cells (ATCC CRL-2266) were fixed and permeabilized using BD Cytofix/Cytoperm™ Fixation/Permeablization Kit (Cat. No. 554714), washed twice with BD Perm/Wash™ Buffer (Cat. No. 554723) , then stained with either Purified Mouse Anti-Human Chromogranin A or Purified Mouse IgG1, κ Isotype Control (Cat. No.555746) monoclonal antibodies followed by PE Goat Anti-Mouse Ig (Multiple Adsorption) (Cat. No. 550589) second-step reagent. The fixed cells were resuspended in stain buffer and acquired on a BD LSR™ II

    BD Pharmingen™ Purified Mouse Anti-Human Chromogranin A - Purified - Clone S21-537 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Human Chromogranin A Recombinant Protein
    Clone
    S21
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product CHGA Primary Antibody
  • Application Notes
    Flow Cytometry System. The dotted line shows isotype control background staining, while the solid line represents Chromogranin A staining. The overlay histograms were derived from gated events with forward and side light-scatter characteristics of viable cells. Additionally, Purified Mouse Anti-Human Chromogranin A was negative on human embryonic stem (ES) cells (data not shown). Immunofluorescent staining of Chromogranin A in human neuroblastoma cells. SH-SY5Y neuroblastoma cells (ATCC CRL-2266) were fixed with BD Cytofix™ Fixation Buffer , permeabilized with BD Perm/Wash™ Buffer and stained with Purified Mouse Anti-Human Chromogranin A monoclonal antibody (pseudo-colored green) at 1.2 μg/mL. The second-step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies), and counter staining was with DAPI (pseudo-colored blue). No Chromogranin A staining was observed on human embryonic stem cells (hESC) that were imaged similarly (data not shown). Also, no staining was observed when SH-SY5Y and hESC were stained with second-step reagent alone (data not shown). The image was captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ Software. BD Phosflow™ Perm Buffer III or Triton™ X-100 are also suitable for permeabilization. Western Blot analysis of Chromogranin A in human SH-SY5Y neuroblastoma lysate. SH-SY5Y neuroblastoma (ATCC CRL-2266) cell lysate was probed with Purified Mouse Anti-Human Chromogranin A monoclonal antibody at titrations of 4.0 to 0.007 μg/lane. Data shown in lanes 1,2,3 are two fold dilutions, 0.25 μg, 0.125 μg, 0.063 μg. Chromogranin A is identified as a band of ~70 kDa. 564562 Rev. 1 Page 1 of 2
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store undiluted at 4°C.
  • Loh, Cheng, Mahata, Corti, Tota: "Chromogranin A and derived peptides in health and disease." in: Journal of molecular neuroscience : MN, Vol. 48, Issue 2, pp. 347-56, (2012) (PubMed).

    Schulz, Young, Agulnick, Babin, Baetge, Bang, Bhoumik, Cepa, Cesario, Haakmeester, Kadoya, Kelly, Kerr, Martinson, McLean, Moorman, Payne, Richardson, Ross, Sherrer, Song, Wilson, Brandon, Green et al.: "A scalable system for production of functional pancreatic progenitors from human embryonic stem cells. ..." in: PLoS ONE, Vol. 7, Issue 5, pp. e37004, (2012) (PubMed).

    Kroon, Martinson, Kadoya, Bang, Kelly, Eliazer, Young, Richardson, Smart, Cunningham, Agulnick, DAmour, Carpenter, Baetge: "Pancreatic endoderm derived from human embryonic stem cells generates glucose-responsive insulin-secreting cells in vivo." in: Nature biotechnology, Vol. 26, Issue 4, pp. 443-52, (2008) (PubMed).

    DAmour, Bang, Eliazer, Kelly, Agulnick, Smart, Moorman, Kroon, Carpenter, Baetge: "Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells." in: Nature biotechnology, Vol. 24, Issue 11, pp. 1392-401, (2006) (PubMed).

    Mouland, Bevan, White, Hendy: "Human chromogranin A gene. Molecular cloning, structural analysis, and neuroendocrine cell-specific expression." in: The Journal of biological chemistry, Vol. 269, Issue 9, pp. 6918-26, (1994) (PubMed).

  • Target
    Chromogranin A (CHGA)
    Alternative Name
    Chromogranin A (CHGA Products)
    Synonyms
    CGA antibody, CG-ALPHA antibody, FSHA antibody, GPHA1 antibody, GPHa antibody, HCG antibody, LHA antibody, TSHA antibody, ChrA antibody, fj05f09 antibody, si:dkey-177p2.2 antibody, wu:fj05f09 antibody, zgc:101749 antibody, zgc:56075 antibody, CHGA antibody, cgA antibody, chga antibody, chromogranin A antibody, glycoprotein hormones, alpha polypeptide antibody, uncharacterized CHGA antibody, chromogranin A S homeolog antibody, CHGA antibody, CGA antibody, Chga antibody, chga antibody, chga.S antibody
    Background
    Synonyms: CGA, CgA, CHGA, Chromogranin-A, CMGA, SP-I
    Pathways
    Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling
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