Fcmu Receptor antibody (Biotin)
Reactivity: Human
FACS
Host: Mouse
Monoclonal
HM14
Biotin
(5 references)-
- Target
- Fcmu Receptor
- Reactivity
- Human
- Host
- Mouse
- Clonality
- Monoclonal
- Conjugate
- Biotin
- Application
- Flow Cytometry (FACS)
- Brand
- BD Pharmingen™
- Characteristics
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The HM14-1 monoclonal antibody specifically binds to the FAIM3-encoded high affinity receptor for IgM (also known as, Fcμ Receptor, FcμR, Fc mu receptor, Immunoglobulin mu Fc receptor, FcmR, IgM Fc receptor). The FcμR is a type 1 transmembrane glycoprotein that contains an extracellular Ig-like domain with sequence similarities to Fcα/μR and pIgR. It is likewise known as Fas apoptotic inhibitory molecule 3, TOSO or Regulator of Fas-induced apoptosis Toso based on its capacity to counter apoptosis mediated by Fas/CD95 (or TNF) in some experimental model systems. FcμRs are expressed by B cells, CD4+ T cells, CD8+ T cells, and NK cells and are highly expressed on Chronic Lymphocytic Leukemia cells. FcμRs may play roles in cellular activation, antigen presentation and IgM-dependent cell-mediated cytotoxicity. The relatively long cytoplasmic tail of FcμR contains several conserved tyrosine and serine residues. These get phosphorylated after FcμR ligation with IgM immune complexes and may initiate signaling responses. Lymphocyte FcμRs can mediate the internalization of IgM-bound antigens including microbial and viral antigens. This in turn may cause cellular activation due to subsequent antigen processing and exposure of activating ligands to intracellular receptors including Toll-like receptors. The HM7-10 antibody, a related FcμR-specific antibody, reportedly recognizes an epitope near the IgM ligand binding site of the FcμR whereas the HM14-1 antibody does not. Two-color flow cytometric analysis of Fcμ Receptor expression on human peripheral blood mononuclear cells. PBMC were stained with Biotin Mouse Anti-Human Fcμ Receptor (Cat. No. 563018), PerCP-Cy™5.5 Mouse Anti-Human CD3 (560835), and APC-Cy™7 Mouse Anti-Human CD11b (557754/560914) antibodies followed by PE Streptavidin (Cat. No. 554061). Two-color flow cytometric dot plots show the correlated expression patterns of CD3 (Left Panel) or CD11b (Right Panel) versus Fcμ Receptor for gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
BD Pharmingen™ Biotin Mouse anti-Human Fcμ Receptor - Biotin - Clone HM14-1 (also known as HM14) - Isotype Mouse IgG1, κ - Reactivity Hu - 50 μg - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- Human IgM Fc Receptor Transfected Cell Line
- Clone
- HM14
- Isotype
- IgG1 kappa
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
- Storage
- 4 °C
- Storage Comment
- Store undiluted at 4°C.
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: "TOSO, the Fcmicro receptor, is highly expressed on chronic lymphocytic leukemia B cells, internalizes upon IgM binding, shuttles to the lysosome, and is downregulated in response to TLR activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 8, pp. 4040-50, (2011) (PubMed).
: "A conserved host and pathogen recognition site on immunoglobulins: structural and functional aspects." in: Advances in experimental medicine and biology, Vol. 946, pp. 87-112, (2011) (PubMed).
: "Identification of TOSO/FAIM3 as an Fc receptor for IgM." in: International immunology, Vol. 22, Issue 3, pp. 149-56, (2010) (PubMed).
: "Identity of the elusive IgM Fc receptor (FcmuR) in humans." in: The Journal of experimental medicine, Vol. 206, Issue 12, pp. 2779-93, (2009) (PubMed).
: "Toso, a cell surface, specific regulator of Fas-induced apoptosis in T cells." in: Immunity, Vol. 8, Issue 4, pp. 461-71, (1998) (PubMed).
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: "TOSO, the Fcmicro receptor, is highly expressed on chronic lymphocytic leukemia B cells, internalizes upon IgM binding, shuttles to the lysosome, and is downregulated in response to TLR activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 8, pp. 4040-50, (2011) (PubMed).
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- Target
- Fcmu Receptor
- Background
- Synonyms: FcμR, FcmR, Fc mu receptor, IgM Fc receptor, FAIM3, TOSO
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