H2-M antibody

Catalog No. ABIN2689548

Product Details

Target: H2-M

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Application: Intracellular Staining (ICS), Immunoprecipitation (IP)

Brand: BD Pharmingen™

Characteristics: H2-M, also known as H2-DM, is a non-classical MHC class II molecule in antigen-presenting cells. H2-M and CD74 (Ii) are critical components of the antigen-processing pathway of the classical MHC class II molecules. Like classical MHC class II molecules, non-classical MHC molecules have limited polymorphism. There are two isoforms of H2-M, αβ1 and αβ2, encoded by the H2-DMa gene and either H2-DMb1 or H2-DMb2, respectively. The 2E5A antibody reacts with H2-M αβ2 dimers (the predominant form transcribed in the mouse spleen), but not αβ1 dimers. H2-M dimers (both isoforms) are integral proteins of lysomal membranes, where they catalyze the release of CLIP (class II-associated Ii peptide) from the peptide-binding groove of classical MHC class II dimers and stabilize the open binding site to allow loading of exogenous peptides. They also may facilitate the selection of high-affinity antigenic peptides by allowing the dissociation of poorly fitting non-CLIP peptides to be exchanged for higher affinity peptides. This peptide-exchange function of H2-M is essential for the intrathymic development of the repertoire of CD4+ T lymphocytes and the maturation of humoral and cell-mediated immune responses. H2-M is believed to be expressed in all cells which express classical MHC class II molecules, including peripheral B lymphocytes, macrophages, dendritic cells, thymic cortical epithelium, and some tumor cell lines. Its level of expression is at least partially controlled by CD74 (Ii), whereas its functional activity is negatively regulated by another non-classical MHC class II molecule, H2-O. Two-color analysis of the cytoplasmic expression of H2-M in splenic B lymphocytes and dendritic cells. Fixed and permeabilized C57BL/6 splenocytes were stained with either purified rat IgG1, κ isotype control mAb R3-34 (Cat. No. 553922, top panels) or purified mAb 2E5A (bottom panels) in the presence of Mouse Fc Block™ (purified anti-mouse CD16/CD32 mAb 2.4 G2, Cat. No. 553141/553142), followed by FITC-conjugated anti-rat IgG1 mAb RG11/39.4 (Cat. No. 553892). B lymphocytes were identified by staining with PE-conjugated anti-mouse CD19 mAb 1D3 (Cat. No. 557399/553786, left panels), and dendritic cells were identified with PE-conjugated anti-mouse CD11c mAb HL3 (Cat. No. 557401/553802, right panels). Flow cytometry was performed on a BD FACSCalibur™ System . 552405 Rev. 1 Page 1 of 2

BD Pharmingen™ Purified Rat Anti-Mouse H2-M - Purified - Clone 2E5A - Isotype Rat IgG1, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Recombinant H2-DM protein

Clone: 2E5A

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References

Lankar, Vincent-Schneider, Briken, Yokozeki, Raposo, Bonnerot: "Dynamics of major histocompatibility complex class II compartments during B cell receptor-mediated cell activation." in: The Journal of experimental medicine, Vol. 195, Issue 4, pp. 461-72, (2002) (PubMed).

Alfonso, Han, Williams, Karlsson: "The impact of H2-DM on humoral immune responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 11, pp. 6348-55, (2001) (PubMed).

Honey, Duff, Beers, Brissette, Elliott, Peters, Maric, Cresswell, Rudensky: "Cathepsin S regulates the expression of cathepsin L and the turnover of gamma-interferon-inducible lysosomal thiol reductase in B lymphocytes." in: The Journal of biological chemistry, Vol. 276, Issue 25, pp. 22573-8, (2001) (PubMed).

Lang, Avé, Huerre, Milon, Antoine: "Macrophage subsets harbouring Leishmania donovani in spleens of infected BALB/c mice: localization and characterization." in: Cellular microbiology, Vol. 2, Issue 5, pp. 415-30, (2001) (PubMed).

Walter, Scheuer, Loos, Reichert, Maeurer: "H2-Mbeta 1 and H2-Mbeta 2 heterodimers equally promote clip removal in I-A(q) molecules from autoimmune-prone DBA/1 mice." in: The Journal of biological chemistry, Vol. 276, Issue 14, pp. 11086-91, (2001) (PubMed).

Alfonso, Karlsson: "Nonclassical MHC class II molecules." in: Annual review of immunology, Vol. 18, pp. 113-42, (2000) (PubMed).

Pierre, Shachar, Matza, Gatti, Flavell, Mellman: "Invariant chain controls H2-M proteolysis in mouse splenocytes and dendritic cells." in: The Journal of experimental medicine, Vol. 191, Issue 6, pp. 1057-62, (2000) (PubMed).

Walter, Lingnau, Schmitt, Loos, Maeurer: "MHC class II antigen presentation pathway in murine tumours: tumour evasion from immunosurveillance?" in: British journal of cancer, Vol. 83, Issue 9, pp. 1192-201, (2000) (PubMed).

Walter, Scheuer, Lingnau, Reichert, Schmitt, Loos, Maeurer: "H2-M, a facilitator of MHC class II peptide loading, and its negative modulator H2-O are differentially expressed in response to proinflammatory cytokines." in: Immunogenetics, Vol. 51, Issue 10, pp. 794-804, (2000) (PubMed).

Rodgers, Levitt, Cresswell, Lindahl, Mathis, Monaco, Singer, Ploegh, Bryant: "A nomenclature solution to mouse MHC confusion." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 10, pp. 6294, (1999) (PubMed).

Walter, Loos, Maeurer: "Differential expression of alternative H2-M isoforms in B cells, dendritic cells and macrophages by proinflammatory cytokines." in: Molecular immunology, Vol. 36, Issue 11-12, pp. 733-43, (1999) (PubMed).

Liljedahl, Winqvist, Surh, Wong, Ngo, Teyton, Peterson, Brunmark, Rudensky, Fung-Leung, Karlsson: "Altered antigen presentation in mice lacking H2-O." in: Immunity, Vol. 8, Issue 2, pp. 233-43, (1998) (PubMed).

Fung-Leung, Surh, Liljedahl, Pang, Leturcq, Peterson, Webb, Karlsson: "Antigen presentation and T cell development in H2-M-deficient mice." in: Science (New York, N.Y.), Vol. 271, Issue 5253, pp. 1278-81, (1996) (PubMed).

Target Details

Target: H2-M

Background: Synonyms: H2-DM