H2-M3 antibody

Catalog No. ABIN2689549

Product Details anti-H2-M3 Antibody

Target: H2-M3 (Histocompatibility 2, M Region Locus 3 (H2-M3))

Reactivity: Chemical

Host: Armenian Hamster

Clonality: Monoclonal

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Brand: BD Pharmingen™

Characteristics: The hamster anti-mouse H2-M3 antibody (clone 130) reacts with the H2-M3 major histocompatibility complex (MHC) non-classical class Ib antigen. H2-M3 (M3) associates with β2-microglobulin and is capable of being expressed by most leukocytes. However, due to a lack of endogenous antigens, M3 has been reported to be undetectable on most cells. Its expression is induced by high-affinity N-formylated peptides from mitochondria, Listeria monocytogenes, and Mycobacterium tuberculosis. The induced expression of M3 is most efficient on antigen presenting cells. M3 presents antigen to cytotoxic T lymphocytes and may play a key role in protective immunity against the intracellular bacteria L. monocytogenes and M. tuberculosis. Furthermore, M3 is capable of presenting mitochondrial antigens for intrathymic positive selection of T-cell receptors which recognize those peptides. Upregulation of H2-M3 expression by exogenous N-formylated peptide. C57BL/6 splenocytes were cultured overnight in the absence (left panel) or presence (right panel) of N-

BD Pharmingen™ Purified Hamster Anti-Mouse H2-M3 - Purified - Clone 130 - Isotype Armenian Hamster IgG - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Purified soluble complex of recombinant truncated M3 and beta2-microglobulin

Clone: 130

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-H2-M3 antibody (ABIN2689549)

Chun, Serbina, Nolt, Wang, Chiu, Flynn, Wang: "Induction of M3-restricted cytotoxic T lymphocyte responses by N-formylated peptides derived from Mycobacterium tuberculosis." in: The Journal of experimental medicine, Vol. 193, Issue 10, pp. 1213-20, (2001) (PubMed).

Berg, Princiotta, Irion, Moticka, Dahl, Staerz: "Positive selection of an H2-M3 restricted T cell receptor." in: Immunity, Vol. 11, Issue 1, pp. 33-43, (1999) (PubMed).

Seaman, Pérarnau, Lindahl, Lemonnier, Forman: "Response to Listeria monocytogenes in mice lacking MHC class Ia molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 9, pp. 5429-36, (1999) (PubMed).

Lindahl, Byers, Dabhi, Hovik, Jones, Smith, Wang, Xiao, Yoshino: "H2-M3, a full-service class Ib histocompatibility antigen." in: Annual review of immunology, Vol. 15, pp. 851-79, (1997) (PubMed).

Shawar, Vyas, Rodgers, Rich: "Antigen presentation by major histocompatibility complex class I-B molecules." in: Annual review of immunology, Vol. 12, pp. 839-80, (1994) (PubMed).

Target Details for H2-M3

Target: H2-M3 (Histocompatibility 2, M Region Locus 3 (H2-M3))

Alternative Name: H2-M3

Target Type: Chemical