BrdU antibody (FITC)

Catalog No. ABIN272340

Product Details anti-BrdU Antibody (FITC)

Target: BrdU (Bromodeoxyuridine (BrdU))

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This BrdU antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Immunogen: Bromodeoxyuridine conjugated to Helix Pomatia Haemocyanin used to immunize BALB/c mice

Clone: MoBu-1

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry. Flow Cytometry (1-5 μg/10^6 cells). This antibody is also useful for detecting proliferating cells by Flow cytometry or Immunofluorescence staining. The FITC conjugate is particularly useful in these applications as a secondary antibody and not necessary for visualization. Also, a specific anti-FITC antibody conjugate can be used for signal amplification if desired.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.08 % Sodium Azide as a preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf Life: One year from despatch.

Expiry Date: 12 months

Target Details for BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Abstract: BrdU Products

Target Type: Chemical

Background: Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. Previous methods of detecting DNA included the use of [3H]-thymidine which would be incorporated into the DNA and could then the DNA could be quantified by autoradiography or scintillation counting. These methods are more difficult and require more cleanup due to the radioactive material. An immunohistochemical assay provides a much simpler and cleaner method for detecting DNA in cells.