OLIG2 antibody (N-Term)

Catalog No. ABIN2746971

Product Details anti-OLIG2 Antibody

Target: OLIG2 (Oligodendrocyte Lineage Transcription Factor 2 (OLIG2))

Binding Specificity: AA 100-150, N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OLIG2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Specificity: The antibody labels a strong band of Olig2 at 40 kDa in ABIN1741657 samples and in other cancer cell lines.

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Does not cross react to any other cellular protein.

Characteristics: Olig2- selective antibodies were generated against a peptide taken from the N-terminal region of the human protein. The Olig2-selective antibodies are affinity purified on an immobilized antigen based affinity matrix, the isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage. The anti- Olig2-selective antibodies are fully characterized for applications in western blotting and ELISA at the recommended dilutions. The supplier Provides Olig2 Western blot positive control samples in ready-to-use SDS-PAGE sample buffer. Limited quantities of antigenic blocking peptide for Fyn antibody is also available, please inquire before ordering.

Purification: Affinity Purified

Immunogen: Synthetic peptide taken within amino acid region 100-150 on human Olig2 protein.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Synonyms: protein kinase C-binding protein RACK17, Basic helix loop helix protein class B 1, BHLHB1, OLIGO2, Oligodendrocyte specific bHLH transcription factor 2, Oligodendrocyte transcription factor 2, PRKCBP2, Protein kinase C binding protein 2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.65 μg/μL

Handling Advice: Working solutions of antibodies in buffer should be filtered through 0.45 μm filter after every use for long-term storage.

Storage: -20 °C

Storage Comment: For long-term storage of keep at -20 °C. We don't recommend storage of very dilute antibody solutions unless they are prepared in specially formulated multi use antibody dilution buffer.

Target Details for OLIG2

Target: OLIG2 (Oligodendrocyte Lineage Transcription Factor 2 (OLIG2))

Alternative Name: Olig2 (OLIG2 Products)

Synonyms: olig3.2 antibody, MGC89336 antibody, OLIG2 antibody, BHLHB1 antibody, OLIGO2 antibody, PRKCBP2 antibody, RACK17 antibody, bHLHe19 antibody, wu:fc64g09 antibody, AI604895 antibody, Bhlhb1 antibody, Olg-2 antibody, Oligo2 antibody, RK17 antibody, oligodendrocyte transcription factor 4 antibody, oligodendrocyte transcription factor 2 antibody, oligodendrocyte lineage transcription factor 2 antibody, olig4 antibody, OLIG2 antibody, Olig2 antibody, olig2 antibody

Background: Oligodendorocytes are the cells that make and maintain central nervous system myelin. The oligodendrocyte lineage-specific basic helix-loop-helix (OLIG) families of transcription factors include OLIG1, OLIG2 and OLIG3, which differ in tissue expression. OLIG proteins are candidates for molecular markers of human glial brain tumors, which are the most common primary malignancies of the human brain. Oligodendrocyte formation in the adult brain is associated with glial-restricted progenitor cells, known as oligodendrocyte progenitor cells (OPCs). Injury to the oligodendroglial cells causes demyelinating diseases such as multiple sclerosis and leukodystrophies. Oligodendrocyte 2 is a transcription factor which is required for oligodendrocyte and motor neuron specification in the spinal cord and for the development of somatic motor neurons in the hindbrain. It cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. It is an antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development. The restricted expression of Olig2 in oligodendrocytes makes AB9610 an excellent marker of oligodendroglia and oligodendrogliomas in development and mature systems. The gene is involved in a chromosomal translocation t (14, 21) (q11.2,q22) associated with T-cell acute lymphoblastic leukemia. Its chromosomal location is within a region of chromosome 21 which plays a role in learning deficits associated with Down syndrome. Its over-expression inhibits neural progenitor proliferation through changes in potassium channel activity, thus contributing to the reduced neuronal numbers and brain size in Down syndrome. Olig2 may be a cause of a form of T-cell acute lymphoblastic leukemia. Interactions between OLIG proteins and Nkx-2.2 promote the formation of alternate cell types by inhibiting V3 interneuron development. Olig2 is useful in recognizing normal Oligodendorocytes on paraffin sections, and applicable in diagnosis of some brain tumors. It is strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas is highly variable. OLIG2, a regulator of white matter development and a candidate gene for schizophrenia, is also associated with psychotic symptoms in Alzheimer's disease.

UniProt: Q13516