Recombinant OLIG2 antibody (AA 200-300)

Catalog No. ABIN7504157

This Rabbit Monoclonal antibody specifically detects OLIG2 in IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant OLIG2 antibody (AA 200-300) (ABIN7504157)

Target: OLIG2 (Oligodendrocyte Lineage Transcription Factor 2 (OLIG2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This OLIG2 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: OLIG2-6695R

Product Details anti-OLIG2 Antibody

Binding Specificity: AA 200-300

Purpose: Recombinant OLIG2 (Marker of Glial Brain Tumors) Antibody

Specificity: Olig2, a basic helix loop helix transcription factor, is involved in oligo-dendroglial specification. Olig2 expression has been reported in most glial tumors, such as oligodendrogliomas and astrocytomas. Although more than half of glioblastomas are positive for Olig2, expression is very weak in terms of both percentage of labeled cells and intensity. No Olig2 expression has been found in the non-glial tumors including neuroepithelial tumors, ependymomas, sub-ependymomas, medulloblastomas, and non-neuroepithelial tumors, such as CNS lymphomas, meningiomas, schwannomas, atypical teratoid/rhabdoid tumor, and haemangioblastomas. Compared to the strong staining seen in glioma samples, a weak expression is observed in non-tumoral brain tissue.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa200-300) of human OLIG2 protein (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes:

Positive Control: THP-1 cells. Human brain or astrocytoma.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for OLIG2

Target: OLIG2 (Oligodendrocyte Lineage Transcription Factor 2 (OLIG2))

Alternative Name: OLIG2

Background:

Basic helix loop helix protein class B1 (bHLHB1), basic helix-loop-helix protein 19 (bHLHe19), OLIG2, Oligodendrocyte lineage transcription factor 2, Oligodendrocyte specific bHLH transcription factor 2, Oligodendrocyte transcription factor 2, Protein kinase C-binding protein 2 (PRKCBP2), RACK17

THP-1 cells. Human brain or astrocytoma.

Molecular Weight: 32kDa

Gene ID: 10215, 176977

UniProt: Q13516