FCGR1A antibody

Catalog No. ABIN2749033

Product Details anti-FCGR1A Antibody

Target: FCGR1A (Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This FCGR1A antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: The mouse monoclonal antibody 10.1 recognizes an extracellular epitope on CD64/FcgammaRI, a 72 kDa single chain type I glycoprotein, that is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The epitope is sensitive to formalin fixation.

Cross-Reactivity (Details): Human, Non-Human Primates

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Endotoxin Level: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: Rheumatoid synovial fluid cells and fibronectin purified human monocytes

Clone: 10-1

Isotype: IgG1 kappa

Application Details

Application Notes: Functional application: Blocking of IgG binding to the FcγR1.
Flow cytometry: Recommended dilution: 1-4 μg/mL.
Immunohistochemistry (paraffin sections and frozen sections): There can occur problems with paraformaldehyde fixation.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-FCGR1A antibody (ABIN2749033)

Devaraj, Davis, Simon, Jialal: "CRP promotes monocyte-endothelial cell adhesion via Fcgamma receptors in human aortic endothelial cells under static and shear flow conditions." in: American journal of physiology. Heart and circulatory physiology, Vol. 291, Issue 3, pp. H1170-6, (2006) (PubMed).

Devaraj, Du Clos, Jialal: "Binding and internalization of C-reactive protein by Fcgamma receptors on human aortic endothelial cells mediates biological effects." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 25, Issue 7, pp. 1359-63, (2005) (PubMed).

Roura-Mir, Wang, Cheng, Matsunaga, Dascher, Peng, Fenton, Kirschning, Moody: "Mycobacterium tuberculosis regulates CD1 antigen presentation pathways through TLR-2." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 3, pp. 1758-66, (2005) (PubMed).

Beekman, Bakema, van der Linden, Tops, Hinten, van Vugt, van de Winkel, Leusen: "Modulation of FcgammaRI (CD64) ligand binding by blocking peptides of periplakin." in: The Journal of biological chemistry, Vol. 279, Issue 32, pp. 33875-81, (2004) (PubMed).

Sánchez-Torres, García-Romo, Cornejo-Cortés, Rivas-Carvalho, Sánchez-Schmitz: "CD16+ and CD16- human blood monocyte subsets differentiate in vitro to dendritic cells with different abilities to stimulate CD4+ T cells." in: International immunology, Vol. 13, Issue 12, pp. 1571-81, (2001) (PubMed).

Brichard, Varis, Latinne, Deneys, de Bruyere, Leveugle, Cornu: "Intracellular cytokine profile of cord and adult blood monocytes." in: Bone marrow transplantation, Vol. 27, Issue 10, pp. 1081-6, (2001) (PubMed).

Fadlon, Vordermeier, Pearson, Mire-Sluis, Dumonde, Phillips, Fishlock, Brown: "Blood polymorphonuclear leukocytes from the majority of sickle cell patients in the crisis phase of the disease show enhanced adhesion to vascular endothelium and increased expression of CD64." in: Blood, Vol. 91, Issue 1, pp. 266-74, (1998) (PubMed).

Hashimoto, Yamada, Motoyoshi, Akagawa: "Enhancement of macrophage colony-stimulating factor-induced growth and differentiation of human monocytes by interleukin-10." in: Blood, Vol. 89, Issue 1, pp. 315-21, (1997) (PubMed).

Jayaram, Buckle, Hogg: "The Fc receptor, FcRI, and other activation molecules on human mononuclear phagocytes after treatment with interferon-gamma." in: Clinical and experimental immunology, Vol. 75, Issue 3, pp. 414-20, (1989) (PubMed).

Dougherty, Selvendran, Murdoch, Palmer, Hogg: "The human mononuclear phagocyte high-affinity Fc receptor, FcRI, defined by a monoclonal antibody, 10.1." in: European journal of immunology, Vol. 17, Issue 10, pp. 1453-9, (1987) (PubMed).

Target Details for FCGR1A

Target: FCGR1A (Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A))

Alternative Name: CD64 (FCGR1A Products)

Background: Fc fragment of IgG receptor Ia,CD64 (FcgammaRI) is a cell surface receptor for Fc region of IgG. It is composed of specific ligand binding alpha subunit and promiscuous gamma subunit, which is indispensable for tyrosine-based signaling. However, even the alpha subunit can transduce signals leading to cellular effector functions. The isoform FcgammaRIa1 binds human IgG with high affinity, has limited myeloid cell distribution, and a relatively large intracellular domain. Products of related genes include FcgammaRIb and FcgammaRIc isoforms, but these specify low affinity IgG receptors if functionally expressed at all. Besides a role in antigen clearance, FcgammaRI (a1) can potently enhance MHC class I and II antigen presentation in vitro and in vivo.,FcRI, IGFR1, FcgR1A

Gene ID: 2209

UniProt: P12314

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process