LAMP2 antibody (FITC)

Catalog No. ABIN2749100

Product Details anti-LAMP2 Antibody (FITC)

Target: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LAMP2 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Purpose: Anti-Hu CD107b FITC

Specificity: The mouse monoclonal antibody H4B4 recognizes an extracellular/luminal epitope of CD107b / LAMP-2, an extensively glycosylated 100-120 kDa widely expressed lysosome-associated protein.

No Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Human PBMC

Clone: H4B4

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular and extracellular staining.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-LAMP2 antibody (FITC) (ABIN2749100)

Meade, Wilson, Holmes, de Wynter, Brett, Straszynski, Ballard, Trapani, McDermott, Cook: "Proteolytic activation of the cytotoxic phenotype during human NK cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 2, pp. 803-13, (2009) (PubMed).

Thedrez, Harly, Morice, Salot, Bonneville, Scotet: "IL-21-mediated potentiation of antitumor cytolytic and proinflammatory responses of human V gamma 9V delta 2 T cells for adoptive immunotherapy." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 6, pp. 3423-31, (2009) (PubMed).

Guia, Cognet, de Beaucoudrey, Tessmer, Jouanguy, Berger, Filipe-Santos, Feinberg, Camcioglu, Levy, Al Jumaah, Al-Hajjar, Stephan, Fieschi, Abel, Brossay, Casanova, Vivier: "A role for interleukin-12/23 in the maturation of human natural killer and CD56+ T cells in vivo." in: Blood, Vol. 111, Issue 10, pp. 5008-16, (2008) (PubMed).

Apte, Baz, Groves, Kelso, Kienzle: "Interferon-gamma and interleukin-4 reciprocally regulate CD8 expression in CD8+ T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 45, pp. 17475-80, (2008) (PubMed).

Kannanganat, Ibegbu, Chennareddi, Robinson, Amara: "Multiple-cytokine-producing antiviral CD4 T cells are functionally superior to single-cytokine-producing cells." in: Journal of virology, Vol. 81, Issue 16, pp. 8468-76, (2007) (PubMed).

Palmer, Fernandez, Bisbing, Peachman, Rao, Barvir, Gunther, Burgess, Kohno, Padmanabhan, Sun: "Restricted replication and lysosomal trafficking of yellow fever 17D vaccine virus in human dendritic cells." in: The Journal of general virology, Vol. 88, Issue Pt 1, pp. 148-56, (2006) (PubMed).

Denzer, van Eijk, Kleijmeer, Jakobson, de Groot, Geuze: "Follicular dendritic cells carry MHC class II-expressing microvesicles at their surface." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 3, pp. 1259-65, (2000) (PubMed).

Target Details for LAMP2

Target: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Alternative Name: CD107b (LAMP2 Products)

Background: Lysosomal associated membrane protein 2,CD107b (lysosome-associated membrane protein-2, LAMP-2), together with CD107a / LAMP-1, is a major constituent of lysosomal membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes, especially CD107b is important regulator in successful phagosomal maturation. CD107b deficiency causes an accumulation of autophagosomes in many tissues leading to cardiomyopathy and myopathy (Danons disease). Immature CD107b is an approximately 45 kDa protein, but after extensive glycosylation the mature glycoprotein has about 100-120 kDa.,LAMP-2, LAMPB

Gene ID: 3920

UniProt: P13473

Pathways: Autophagy