PLAP antibody (C-Term)

Catalog No. ABIN2777088

Product Details anti-PLAP Antibody

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: TVLLYGNGPG YVLKDGARPD VTESESGSPE YRQQSAVPLD EETHAGEDVA

Predicted Reactivity: Cow: 86%, Dog: 86%, Guinea Pig: 79%, Horse: 79%, Human: 100%, Mouse: 79%, Rabbit: 79%, Rat: 79%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against ALPP. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human ALPP

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 535 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-PLAP antibody (ABIN2777088)

Lazrek, Goffard, Schanen, Karquel, Bocket, Lion, Devaux, Hedouin, Gosset, Hober: "Detection of hepatitis C virus antibodies and RNA among medicolegal autopsy cases in Northern France." in: Diagnostic microbiology and infectious disease, Vol. 55, Issue 1, pp. 55-8, (2006) (PubMed).

Target Details for PLAP

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Alternative Name: ALPP (ALPP Products)

Background: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. ALPP is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form.There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized.
Alias Symbols: ALP, PLAP, PALP
Protein Interaction Partner: COL2A1, KRTAP10-3, KRTAP4-12, KRTAP5-9, RPA3, RPA2, RPA1, FBXO6, LATS1, UBC, SUMO1, SUMO2, UBXN6, Cep76, Trim69, Erh, Cbx1, UBXN11, ASPSCR1, NSFL1C, UBXN1, UBXN7, FAF2, FAF1, ALPP, PIGK, GPLD1,
Protein Size: 535

Molecular Weight: 59 kDa

Gene ID: 250

NCBI Accession: NM_001632, NP_001623

UniProt: P05187