MMP 9 antibody (N-Term)

Catalog No. ABIN2777743

Product Details anti-MMP 9 Antibody

Target: MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))

Binding Specificity: N-Term

Reactivity: Human, Rat, Mouse, Rabbit, Cow, Dog, Horse, Guinea Pig, Zebrafish (Danio rerio), Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MMP 9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: VAEHGDGYPF DGKDGLLAHA FPPGPGIQGD AHFDDDELWS LGKGVVVPTR

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against MMP9. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MMP9

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 707 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-MMP 9 antibody (ABIN2777743)

Puttabyatappa, Irwin, Martin, Mesquitta, Veiga-Lopez, Padmanabhan: "Developmental Programming: Gestational Exposure to Excess Testosterone Alters Expression of Ovarian Matrix Metalloproteases and Their Target Proteins" in: Reproductive sciences (Thousand Oaks, Calif.), pp. 1933719117697130, (2017) (PubMed).

Strilakou, Perelas, Lazaris, Papavdi, Karkalousos, Giannopoulou, Kriebardis, Panayiotides, Liapi: "Immunohistochemical determination of the extracellular matrix modulation in a rat model of choline-deprived myocardium: the effects of carnitine." in: Fundamental & clinical pharmacology, Vol. 30, Issue 1, pp. 47-57, (2016) (PubMed).

Zhang, Jiang, Zuo: "Pyrrolidine dithiocarbamate attenuates surgery-induced neuroinflammation and cognitive dysfunction possibly via inhibition of nuclear factor κB." in: Neuroscience, Vol. 261, pp. 1-10, (2014) (PubMed).

Li, Tan, Zhang, Han, Di, Xiao, Cheng, Gao, Liu: "DLK1 promotes lung cancer cell invasion through upregulation of MMP9 expression depending on Notch signaling." in: PLoS ONE, Vol. 9, Issue 3, pp. e91509, (2014) (PubMed).

Deng, Zhang, Feng, Xiong, Zuo: "Critical role of matrix metalloprotease-9 in chronic high fat diet-induced cerebral vascular remodelling and increase of ischaemic brain injury in mice†." in: Cardiovascular research, Vol. 103, Issue 4, pp. 473-84, (2014) (PubMed).

Gotschy, Bauer, Schrodt, Lykowsky, Ye, Rommel, Jakob, Bauer, Herold: "Local arterial stiffening assessed by MRI precedes atherosclerotic plaque formation." in: Circulation. Cardiovascular imaging, Vol. 6, Issue 6, pp. 916-23, (2013) (PubMed).

Target Details for MMP 9

Target: MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))

Alternative Name: MMP9 (MMP9 Products)

Background: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
Alias Symbols: GELB, CLG4B, MMP-9, MANDP2
Protein Interaction Partner: CXCL5, PZP, MMP26, RECK, KISS1, PRSS2, EPHB2, BTC, LCN2, COL4A6, COL4A5, TIMP3, THBS2, THBS1, MMP10, MMP7, PLG, CXCL1, TFPI, CXCL8, FN1, MMP9, COL1A2, COL1A1, COL4A4, COL4A1, COL4A2, COL4A3, HAPLN1, CD44, AREG, TGFB1, CXCL6,
Protein Size: 707

Molecular Weight: 78 kDa

Gene ID: 4318

NCBI Accession: NM_004994, NP_004985

UniProt: P14780

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events