KHSRP antibody (Middle Region)

Catalog No. ABIN2778858

Product Details anti-KHSRP Antibody

Target: KHSRP (KH-Type Splicing Regulatory Protein (KHSRP))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KHSRP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: WEEYYKKQAQ VATGGGPGAP PGSQPDYSAA WAEYYRQQAA YYGQTPGPGG

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against KHSRP. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Human KHSRP

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 711 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-KHSRP antibody (ABIN2778858)

Ahn, Hwang, Liu, Wang, Zhang, Stephenson, Boronina, Cole, Dinkova-Kostova, Talalay, Cole: "Electrophilic tuning of the chemoprotective natural product sulforaphane." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 21, pp. 9590-5, (2010) (PubMed).

Target Details for KHSRP

Target: KHSRP (KH-Type Splicing Regulatory Protein (KHSRP))

Alternative Name: KHSRP (KHSRP Products)

Background: KHSRP binds to the dendritic targeting element and may play a role in mRNA trafficking. It is part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. KHSRP mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. It may interact with single-stranded DNA from the far-upstream element (FUSE).It is also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.
Alias Symbols: FBP2, FUBP2, KSRP, MGC99676
Protein Interaction Partner: HUWE1, Bace1, UBC, STAU1, LGR4, MDM2, WWOX, SUZ12, EED, RNF2, NAP1L4, HNRNPD, GFPT1, GARS, APBB1, ALDH16A1, TARDBP, CD81, MPG, UBL4A, WHSC1, VCAM1, ITGA4, FN1, STRIP1, ASF1B, CPLX1, CIRBP, SUMO1, RAE1, CAND1, COPS5, CUL1, CUL2, CUL3, CUL4B, CUL5, NEDD8, C
Protein Size: 711

Molecular Weight: 73 kDa

Gene ID: 8570

NCBI Accession: NM_003685, NP_003676

UniProt: Q92945