SF3A3 antibody (Middle Region)

Catalog No. ABIN2779010

Product Details anti-SF3A3 Antibody

Target: SF3A3 (Splicing Factor 3a, Subunit 3, 60kDa (SF3A3))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Horse, Rabbit, Guinea Pig, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SF3A3 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: THENVQRKQA RTGEEREEEE EEQISESESE DEENEIIYNP KNLPLGWDGK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 100%, Zebrafish: 91%

Characteristics: This is a rabbit polyclonal antibody against SF3A3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SF3A3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 501 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-SF3A3 antibody (ABIN2779010)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for SF3A3

Target: SF3A3 (Splicing Factor 3a, Subunit 3, 60kDa (SF3A3))

Alternative Name: SF3A3 (SF3A3 Products)

Background: SF3A3 is the subunit 3 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 3 interacts with subunit 1 through its amino-terminus while the zinc finger domain of subunit 3 plays a role in its binding to the 15S U2 snRNP.This This gene encodes subunit 3 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 3 interacts with subunit 1 through its amino-terminus while the zinc finger domain of subunit 3 plays a role in its binding to the 15S U2 snRNP. This gene has a pseudogene on chromosome 20. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: PRP9, PRPF9, SAP61, SF3a60
Protein Interaction Partner: TRIM69, HUWE1, ZNF212, TADA2A, FUS, UBC, NEDD8, MDM2, WWOX, RNF2, SUZ12, EED, rev, APBB1, ACTN1, ACTN4, STAM2, HGS, SNRPB, CDAN1, PRPF40A, WBP4, TCERG1, UBD, SF3A1, FN1, gag-pol, PRMT5, PRPF19, PRPF31, SF3B1, SF3B3, SF3B4, HNRNPR, DDX46, TOX4, DDX23, PRPF
Protein Size: 501

Molecular Weight: 59 kDa

Gene ID: 10946

NCBI Accession: NM_006802, NP_006793

UniProt: Q12874

Pathways: Ribonucleoprotein Complex Subunit Organization