SRSF1 antibody (serine/arginine-Rich Splicing Factor 1) (C-Term)

Details for Product anti-SRSF1 Antibody No. ABIN2779013
Antigen
Epitope
C-Term
11
9
4
3
2
2
2
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rat (Rattus), Zebrafish (Danio rerio)
76
42
42
9
9
5
5
4
4
4
4
4
4
3
2
1
1
1
Host
Rabbit
70
6
Clonality
Polyclonal
Conjugate
This SRSF1 antibody is un-conjugated
4
2
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
63
27
22
12
9
9
4
3
3
1
1
1
1
Options
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human SFRS1
Blocking Peptide Blocking peptide for this product available: ABIN983639
Sequence EFVRKEDMTY AVRKLDNTKF RSHEGETAYI RVKVDGPRSP SYGRSRSRSR
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 100%
Characteristics This is a rabbit polyclonal antibody against SFRS1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Antigen
Alternative Name SFRS1 (SRSF1 Antibody Abstract)
Background SFRS1 is a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. The protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. Multiple transcript variants encoding different isoforms have been found for this gene.Alternative mRNA splicing plays an important role in development and differentiation, many transcripts are spliced differently in distinct cell types and tissues. Both constitutive and alternative splicing occurs on spliceosomes, which are complex particles composed of small nuclear ribonucleoproteins (snRNPs) and non-snRNP proteins. The SR family of non-snRNP splicing factors is characterized by the presence of an RNA recognition motif and a serine- and arginine-rich (SR) domain. SR proteins are required at early stages of spliceosome assembly, have distinct but overlapping specificities for different pre-mRNAs, and can alter splice site choice, suggesting that they may be involved in the regulation of alternative splicing in vivo. Two of the SR proteins, ASF/SF2 (SFRS1) and SC35 (SFRS2, MIM 600813), have been extensively characterized.Alternative mRNA splicing plays an important role in development and differentiation, many transcripts are spliced differently in distinct cell types and tissues. Both constitutive and alternative splicing occurs on spliceosomes, which are complex particles composed of small nuclear ribonucleoproteins (snRNPs) and non-snRNP proteins. The SR family of non-snRNP splicing factors is characterized by the presence of an RNA recognition motif and a serine- and arginine-rich (SR) domain. SR proteins are required at early stages of spliceosome assembly, have distinct but overlapping specificities for different pre-mRNAs, and can alter splice site choice, suggesting that they may be involved in the regulation of alternative splicing in vivo. Two of the SR proteins, ASF/SF2 (SFRS1) and SC35 (SFRS2, MIM 600813), have been extensively characterized (Bermingham et al., 1995).[supplied by OMIM].
Alias Symbols: ASF, SF2, SFRS1, SF2p33, SRp30a, SRSF1
Protein Interaction Partner: TRAF3IP2, HUWE1, SPRTN, STAU1, UBC, MDM2, ZBTB1, RNF2, EZH2, EED, SUZ12, PARK2, SRPK2, SRPK1, SRPK3, FBXO6, YWHAE, U2AF2, PAN2, MPG, CIR1, FN1, CDK6, HNRNPA0, RNPS1, SRSF10, NXF1, PRPF4, BCL10, U2AF1, SRSF4, MAGOH, SMURF1, EIF4A3, ESR1, ECT2, BRCA1, BARD1
Protein Size: 248
Molecular Weight 27 kDa
Gene ID 6426
NCBI Accession NM_006924, NP_008855
UniProt Q07955
Pathways Ribonucleoprotein Complex Subunit Organization
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 248 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Image no. 1 for anti-serine/arginine-Rich Splicing Factor 1 (SRSF1) (C-Term) antibody (ABIN2779013) WB Suggested Anti-SFRS1 Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:.2500 Positi...
Image no. 2 for anti-serine/arginine-Rich Splicing Factor 1 (SRSF1) (C-Term) antibody (ABIN2779013) WB Suggested Anti-SFRS1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Posi...
Product cited in: Li, Xia, Huang, Chen, Su, Li, Wang, Ding, Shao: "A strategy to rapidly identify the functional targets of microRNAs by combining bioinformatics and mRNA cytoplasmic/nucleic ratios in culture cells." in: FEBS letters, Vol. 584, Issue 14, pp. 3198-202, 2010 (PubMed).