TAF15 antibody (N-Term)

Catalog No. ABIN2779283

Product Details anti-TAF15 Antibody

Target: TAF15 (TAF15 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 68kDa (TAF15))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Dog, Guinea Pig, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TAF15 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: TDSSYGQNYS GYSSYGQSQS GYSQSYGGYE NQKQSSYSQQ PYNNQGQQQN

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%, Yeast: 77%

Characteristics: This is a rabbit polyclonal antibody against TAF15. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TAF15

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 592 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-TAF15 antibody (ABIN2779283)

Schatz, Brändlein, Rückl, Hensel, Vollmers: "Diagnostic and therapeutic potential of a human antibody cloned from a cancer patient that binds to a tumor-specific variant of transcription factor TAF15." in: Cancer research, Vol. 70, Issue 1, pp. 398-408, (2010) (PubMed).

Lee, Kim, Pelletier, Kim: "Stimulation of hTAFII68 (NTD)-mediated transactivation by v-Src." in: FEBS letters, Vol. 564, Issue 1-2, pp. 188-98, (2004) (PubMed).

Target Details for TAF15

Target: TAF15 (TAF15 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 68kDa (TAF15))

Alternative Name: TAF15 (TAF15 Products)

Background: Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. Its gene encodes a subunit of TFIID present in a subset of TFIID complexes. Translocations involving chromosome 17 and chromosome 9, where the gene for the nuclear receptor CSMF is located, result in a gene fusion product that is an RNA binding protein associated with a subset of extraskeletal myxoid chondrosarcomas.Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a subunit of TFIID present in a subset of TFIID complexes. Translocations involving chromosome 17 and chromosome 9, where the gene for the nuclear receptor CSMF is located, result in a gene fusion product that is an RNA binding protein associated with a subset of extraskeletal myxoid chondrosarcomas. Two transcripts encoding different isoforms have been identified.
Alias Symbols: Npl3, RBP56, TAF2N, TAFII68, hTAFII68
Protein Interaction Partner: RPA3, RPA2, RPA1, SUZ12, RNF2, EZH2, BMI1, EED, UBD, PRMT8, MED26, UBC, FUS, TPR, TPM2, DDB1, SCARB2, RNF168, TMED9, SAP30BP, NEDD8, CAND1, DCUN1D1, COPS5, COPS6, CUL1, CUL2, CUL3, CUL4A, CUL4B, CUL5, YWHAZ, SIRT7, HIST2H4A, HIST1H3A, HIST2H2AC, CACNA1A,
Protein Size: 592

Molecular Weight: 61 kDa

Gene ID: 8148

NCBI Accession: NM_139215, NP_631961