ZFPM1 antibody (Middle Region)

Catalog No. ABIN2779499

Product Details anti-ZFPM1 Antibody

Target: ZFPM1 (Zinc Finger Protein, Multitype 1 (ZFPM1))

Binding Specificity: Middle Region

Reactivity: Human, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZFPM1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: PPPPPPGHAP APESPRPGSG SGSGPGLAPA RSPGPAADGP IDLSKKPRRP

Predicted Reactivity: Cow: 91%, Human: 100%, Rat: 83%

Characteristics: This is a rabbit polyclonal antibody against ZFPM1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ZFPM1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1006 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ZFPM1 antibody (ABIN2779499)

Sugiyama, Tanaka, Kitajima, Zheng, Yen, Murotani, Yamatodani, Nakano: "Differential context-dependent effects of friend of GATA-1 (FOG-1) on mast-cell development and differentiation." in: Blood, Vol. 111, Issue 4, pp. 1924-32, (2008) (PubMed).

Target Details for ZFPM1

Target: ZFPM1 (Zinc Finger Protein, Multitype 1 (ZFPM1))

Alternative Name: ZFPM1 (ZFPM1 Products)

Background: ZFPM1 is a transcription regulator that plays an essential role in erythroid and megakaryocytic cell differentiation. ZFPM1 is an essential cofactor that acts via the formation of a heterodimer with transcription factors of the GATA family GATA1, GATA2 and GATA3. Such heterodimer can both activate or repress transcriptional activity, depending on the cell and promoter context. The heterodimer formed with GATA proteins is essential to activate expression of genes such as NFE2, ITGA2B, alpha- and beta-globin, while it represses expression of KLF1. ZFPM1 may be involved in regulation of some genes in gonads. ZFPM1 may also be involved in cardiac development, in a non-redundant way with ZFPM2/FOG2.
Alias Symbols: FOG, FOG1, ZNF408, ZNF89A
Protein Interaction Partner: GSK3B, CTBP2, TRIM21, MTA1, RBBP4, Rb1, GATA1, GIPC1, TOP3B, GATA2,
Protein Size: 1006

Molecular Weight: 105 kDa

Gene ID: 161882

NCBI Accession: NM_153813, NP_722520

UniProt: Q8IX07