Dynamitin antibody (N-Term)

Catalog No. ABIN2787686

Product Details anti-Dynamitin Antibody

Target: Dynamitin (DCTN2) (Dynactin 2 (p50) (DCTN2))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Dog, Horse, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dynamitin antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: ADPKYADLPG IARNEPDVYE TSDLPEDDQA EFDAFAQELE ELTSTSVEHI

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against DCTN2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DCTN2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 406 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Dynamitin

Target: Dynamitin (DCTN2) (Dynactin 2 (p50) (DCTN2))

Alternative Name: DCTN2 (DCTN2 Products)

Background: DCTN2 is a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place.This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: DCTN50, DYNAMITIN, RBP50
Protein Interaction Partner: KIFC3, CCDC172, HAUS1, BLOC1S6, DCTN2, BICD2, DYNC1I1, DCTN1, UBC, FLNB, NUP43, LEO1, SEH1L, ACTR10, DCTN4, DCTN3, ACTR1A, ACTR1B, RABEP1, LMNB1, GADD45A, KPNA2, SMURF1, DYNLRB1, STRN4, RANBP9, TPM4, SNRPA1, PSMD4, PSMD1, PSMC4, PSMC2, POLR2D, POLR2A, MCM
Protein Size: 406

Molecular Weight: 45 kDa

Gene ID: 10540

NCBI Accession: NM_006400, NP_006391

UniProt: Q13561

Pathways: M Phase, Ribonucleoprotein Complex Subunit Organization