PRNP antibody (Middle Region)

Catalog No. ABIN2789398

Product Details anti-PRNP Antibody

Target: PRNP (Prion Protein (PRNP))

Binding Specificity: Middle Region

Reactivity: Human, Rat, Cow, Sheep, Dog, Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRNP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: GGGTHNQWNK PSKPKTNLKH VAGAAAAGAV VGGLGGYMLG SAMSRPMIHF

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Human: 100%, Rat: 100%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against Prnp. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Mouse Prnp

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PRNP

Target: PRNP (Prion Protein (PRNP))

Alternative Name: Prnp (PRNP Products)

Background: Prnp may play a role in neuronal development and synaptic plasticity, may be required for neuronal myelin sheath maintenance and may play a role in iron uptake and iron homeostasis. Prnp is a soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. It also provides Cu2+ or ZN2+ for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains.
Alias Symbols: AA960666, AI325101, CD230, PrP, PrP, PrPC, PrPSc, Prn-i, Prn-p, Sinc, prP27-30, prP33-35C
Protein Interaction Partner: Dpp6, Eri3, Syn1, Grb2, Opcml, Lsamp, Ntm, Atp1a3, Igsf8, Cadm3, Clstn1, Pde10a, Adam23, Ywhaz, Ywhae, Stxbp1, Plp1, P4hb, Ncam2, Ncam1, Mog, Mbp, Mag, L1cam, Hspa5, Gnb1, Sparcl1, Dnm1, Dpysl2, Cntn1, Clu, Atp2b2, Atp1b1, Atp1a1, App, Apoe, Aplp2, Apbb1,
Protein Size: 254

Molecular Weight: 28 kDa

Gene ID: 19122

NCBI Accession: NM_011170, NP_035300

UniProt: P04925

Pathways: Transition Metal Ion Homeostasis, Activated T Cell Proliferation