Fluorescein antibody

Catalog No. ABIN2801850

Supplier Product No.: 100-1196

Product Details

Target: Fluorescein

Host: Goat

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 100-1196

Supplier: Rockland

Purpose: Fluorescein Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, Fluorescein conjugated goat IgG and Fluorescein conjugated KLH.

Characteristics: Synonyms: goat anti-Fluorescein Antibody, goat anti-FITC antibody

Purification: Fluorescein Antibody was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen:

Immunogen: Fluorescein conjugated Bovine Serum Albumin (BSA)

Immunogen Type: Native Protein

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-Fluorescein Antibody has been assayed against 1.0 μg of Fluorescein conjugated Rabbit IgG in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Donkey) code #605-703-125 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:100,000 to 1:500,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 90 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References

Kotani, Yamaguchi, Ohnishi, Kuwahara, Nakano, Nakano, Ida, Murakoshi, Honke: "Proximity proteomics identifies cancer cell membrane cis-molecular complex as a potential cancer target." in: Cancer science, Vol. 110, Issue 8, pp. 2607-2619, (2019) (PubMed).

Miyagawa-Yamaguchi, Kotani, Honke: "Expressed glycosylphosphatidylinositol-anchored horseradish peroxidase identifies co-clustering molecules in individual lipid raft domains." in: PLoS ONE, Vol. 9, Issue 3, pp. e93054, (2015) (PubMed).

Target Details

Target: Fluorescein

Target Type: Chemical

Background: Background: Anti-Fluorescein is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.