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IL3RA antibody (Interleukin 3 Receptor, alpha (Low Affinity)) (FITC) Primary Antibody

IL3RA Reactivity: Human FACS Host: Mouse Monoclonal 6H6 FITC
Catalog No. ABIN2853580
$550.00
Plus shipping costs $45.00
50 μg
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Available
  • Target
    IL3RA
    Reactivity
    • 88
    • 31
    • 6
    • 1
    Human
    Host
    • 49
    • 42
    • 3
    • 1
    • 1
    Mouse
    Clonality
    • 51
    • 45
    Monoclonal
    Conjugate
    • 53
    • 8
    • 6
    • 6
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This IL3RA antibody is conjugated to FITC
    Application
    • 52
    • 47
    • 22
    • 18
    • 13
    • 13
    • 11
    • 8
    • 8
    • 5
    • 5
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Specificity
    This antibody reacts with Human CD123 antigen (IL-3 receptor alpha-chain).
    Cross-Reactivity (Details)
    Species reactivity (tested):Human.
    Purification
    Protein-A Sepharose Chromatography.
    Immunogen
    COS cells overexpressing IL-3 receptor alpha-chain. Remarks: Hybridoma was established by fusion of mouse myeloma cell NS-1 with Balb/c mouse splenocyte
    Clone
    6H6
    Isotype
    IgG1
  • Application Notes
    Flow Cytometry: 10-20 μg/mL (final concentration). Positive Control: Transfectant cells. Detailed procedure is provided in Protocols.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Protocol
    Flow Cytometric Aanalysis for Floating CellsProtocol 1We use Fisher tubes or equivalents as reaction tubes for all steps described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN3]. 2) Add 10 μL of normal goat serum to the cell pellet after tapping. Mix well, and incubatefor 5 minutes at room temperature (20~25°C). 3) Add 30 µL of the FITC labeled CD123 monoclonal antibody (10-20 μg/mL) diluted with thewashing buffer. Mix well, and incubate for 30 minutes at room temperature (20~25°C). 4) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 5) Resuspend the cells with 500 μL of the washing buffer and analyze by a flow cytometer. Protocol 2We usually use Fisher tubes or equivalents as reaction tubes for all steps described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN3]. 2) Resuspend the cells with PBS containing 25% normal goat serum and 0. 1% NaN3(5x10e6 cells/mL). 3) Add 20 µL of the non diluted FITC labeled CD123 monoclonal antibody into each tube. 4) Add 50 µL of the cell suspension into each tube. Mix well, and incubate for 30 minutesat room temperature (20~25°C). 5) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 6) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Flow Cytometric analysis for whole blood cellsWe usually use Falcon tubes or equivalents as reaction tubes for all steps described below. 1) Add 20 μL of the non diluted FITC labeled CD123 monoclonal antibody into each tube. 2) Add 50 μL of whole blood into each tube. Mix well, and incubate for 30 minutes at roomtemperature (20~25°C). 3) Lyse with OptiLyse C (for analysis on Beckman Coulterinstruments) or OptiLyse B (foranalysis on BD instruments), using the procedure recommended in the respective packageinserts. 4) Resuspend the cells with 500 μL of the washing buffer [PBS containing 2% fetal calfserum (FCS) and 0. 1% NaN3] and analyze by a flow cytometer. Positive Control for Flow Cytometry: Peripheral blood monocytes.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.05 mg/mL
    Buffer
    PBS containing 1 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C.
  • Target
    IL3RA
    Alternative Name
    CD123 / IL3RA (IL3RA Antibody Abstract)
    Synonyms
    CD123, IL3R, IL3RAY, IL3RX, IL3RY, hIL-3Ra, CDw123, SUT-1, Cyrl, interleukin 3 receptor subunit alpha, interleukin 3 receptor, alpha chain, IL3RA, Il3ra
    Background
    CD123 is the IL-3-specific component of the IL-3 receptor. CD123 binds IL-3 only in combination with CDw131, the Synonyms: IL-3R-alpha, IL3 receptor alpha, Interleukin-3 receptor subunit alpha
    Gene ID
    3563
    NCBI Accession
    NP_002174
    UniProt
    P26951
    Pathways
    JAK-STAT Signaling
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