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Nanog Homeobox (NANOG) (Center) antibody Primary Antibody

NANOG Reactivity: Human, Mouse ICC, IF, IP, IHC, ELISA, WB Host: Rabbit Polyclonal
Pubmed (6)
Catalog No. ABIN2854880
$509.08
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100 μL
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  • Target
    Binding Specificity
    Center
    Reactivity
    Human, Mouse
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    Un-conjugated
    Application
    Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
    Characteristics
    Rabbit Polyclonal antibody to Nanog (Nanog homeobox)
    Nanog antibody [N3C3]
    Purification
    Purified by antigen-affinity chromatography.
    Immunogen
    Recombinant protein encompassing a sequence within the center region of human NANOG. The exact sequence is proprietary.
    Isotype
    IgG
  • Application Notes
    Suggested dilution Reference ELISA 1:1000-1:10000* ICC/IF 1:100-1:1000* Immunohistochemistry Assay-dependent dilution Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceELISA1:1000-1:10000* ICC/IF1:100-1:1000* ImmunohistochemistryAssay-dependent dilution Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
    Comment

    Positive Control: HeLa nucleus , NIH-3T3 , mouse ESC

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
    Preservative
    Thimerosal (Merthiolate)
    Precaution of Use
    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Shen, Kang, Shakya, Tabaka, Jarboe, Regev, Tantin: "Enforcement of developmental lineage specificity by transcription factor Oct1." in: eLife, Vol. 6, 2018 (PubMed).

    Hsu, Chang, Hsu, Liu, Yeh, Wang, Liou, Ko, Chang, Chuang: "Suberoylanilide hydroxamic acid represses glioma stem-like cells." in: Journal of biomedical science, Vol. 23, Issue 1, pp. 81, 2017 (PubMed).

    Marinho, Chailangkarn, Muotri: "Systematic optimization of human pluripotent stem cells media using Design of Experiments." in: Scientific reports, Vol. 5, pp. 9834, 2016 (PubMed).

    Ju, Huang, Huang, Su: "Identification of thiostrepton as a novel therapeutic agent that targets human colon cancer stem cells." in: Cell death & disease, Vol. 6, pp. e1801, 2016 (PubMed).

    Singh, Chappell, Trost, Lin, Wang, Tang, Matlock, Weller, Wu, Zhao, Jin, Dalton: "Cell-cycle control of developmentally regulated transcription factors accounts for heterogeneity in human pluripotent cells." in: Stem cell reports, Vol. 1, Issue 6, pp. 532-44, 2015 (PubMed).

    Liu, Bain: "Arsenic inhibits hedgehog signaling during P19 cell differentiation." in: Toxicology and applied pharmacology, Vol. 281, Issue 3, pp. 243-53, 2014 (PubMed).

  • Target
    Alternative Name
    Nanog (NANOG Antibody Abstract)
    Background
    Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.

    Cellular Localization: Nucleus
    Molecular Weight
    35 kDa
    Gene ID
    79923
    Pathways
    Stem Cell Maintenance
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