Nanog antibody

Catalog No. ABIN2854880

Product Details anti-Nanog Antibody

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nanog antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Ceratotherium simum, Human, Mouse

Characteristics: Rabbit Polyclonal antibody to Nanog (Nanog homeobox)
Nanog antibody [N3C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human NANOG. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. ELISA: 1:1000-1:10000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLa nucleus

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-Nanog antibody (ABIN2854880)

Shen, Kang, Shakya, Tabaka, Jarboe, Regev, Tantin: "Enforcement of developmental lineage specificity by transcription factor Oct1." in: eLife, Vol. 6, (2018) (PubMed).

Hsu, Chang, Hsu, Liu, Yeh, Wang, Liou, Ko, Chang, Chuang: "Suberoylanilide hydroxamic acid represses glioma stem-like cells." in: Journal of biomedical science, Vol. 23, Issue 1, pp. 81, (2017) (PubMed).

Marinho, Chailangkarn, Muotri: "Systematic optimization of human pluripotent stem cells media using Design of Experiments." in: Scientific reports, Vol. 5, pp. 9834, (2016) (PubMed).

Ju, Huang, Huang, Su: "Identification of thiostrepton as a novel therapeutic agent that targets human colon cancer stem cells." in: Cell death & disease, Vol. 6, pp. e1801, (2016) (PubMed).

Singh, Chappell, Trost, Lin, Wang, Tang, Matlock, Weller, Wu, Zhao, Jin, Dalton: "Cell-cycle control of developmentally regulated transcription factors accounts for heterogeneity in human pluripotent cells." in: Stem cell reports, Vol. 1, Issue 6, pp. 532-44, (2015) (PubMed).

Liu, Bain: "Arsenic inhibits hedgehog signaling during P19 cell differentiation." in: Toxicology and applied pharmacology, Vol. 281, Issue 3, pp. 243-53, (2014) (PubMed).

Target Details for Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Alternative Name: Nanog homeobox (NANOG Products)

Synonyms: 2410002E02Rik antibody, ENK antibody, ecat4 antibody, NANOG antibody, hacp antibody, wu:fd19e04 antibody, wu:fd20a07 antibody, zgc:193933 antibody, Nanog homeobox antibody, nanog homeobox antibody, Nanog antibody, NANOG antibody, nanog antibody

Background: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.

Cellular Localization: Nucleus

Molecular Weight: 35 kDa

Gene ID: 79923

UniProt: Q9H9S0

Pathways: Stem Cell Maintenance