ZC3H12A antibody

Catalog No. ABIN2856778

Product Details anti-ZC3H12A Antibody

Target: ZC3H12A (Zinc Finger CCCH-Type Containing 12A (ZC3H12A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZC3H12A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: Knockdown/Knockout validation was supported by references (PMID:27322373)

Cross-Reactivity: Human

Characteristics: Rabbit Polyclonal antibody to ZC3H12A (zinc finger CCCH-type containing 12A)
ZC3H12A antibody [N3C3]

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the center region of human ZC3H12A. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Validatio: KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.8 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-ZC3H12A antibody (ABIN2856778)

Li, Huang, Huang, Li, Falcon, Soutar, Bornancin, Jiang, Xin, Fu: "Pharmacological inhibition of MALT1 protease activity suppresses endothelial activation via enhancing MCPIP1 expression." in: Cellular signalling, Vol. 50, pp. 1-8, (2019) (PubMed).

Li, Huang, Huang, He, Lei, Saaoud, Yu, Melnick, Kumar, Papasian, Fan, Fu: "Central role of myeloid MCPIP1 in protecting against LPS-induced inflammation and lung injury." in: Signal transduction and targeted therapy, Vol. 2, pp. 17066, (2017) (PubMed).

Skalniak, Dziendziel, Jura: "MCPIP1 contributes to the toxicity of proteasome inhibitor MG-132 in HeLa cells by the inhibition of NF-κB." in: Molecular and cellular biochemistry, Vol. 395, Issue 1-2, pp. 253-63, (2015) (PubMed).

Skalniak, Koj, Jura: "Proteasome inhibitor MG-132 induces MCPIP1 expression." in: The FEBS journal, Vol. 280, Issue 11, pp. 2665-74, (2013) (PubMed).

Qi, Liang, She, Cai, Wang, Lei, Stallcup, Fu: "MCP-induced protein 1 suppresses TNFalpha-induced VCAM-1 expression in human endothelial cells." in: FEBS letters, Vol. 584, Issue 14, pp. 3065-72, (2010) (PubMed).

Target Details for ZC3H12A

Target: ZC3H12A (Zinc Finger CCCH-Type Containing 12A (ZC3H12A))

Alternative Name: zinc finger CCCH-type containing 12A (ZC3H12A Products)

Background: ZC3H12A is an MCP1 (CCL2, MIM 158105)-induced protein that acts as a transcriptional activator and causes cell death of cardiomyocytes, possibly via induction of genes associated with apoptosis.[supplied by OMIM]

Cellular Localization: Cytoplasm (By similarity) , Nucleus

Molecular Weight: 66 kDa

Gene ID: 80149

UniProt: Q5D1E8

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of fat Cell Differentiation