CD86 antibody (CD86 Molecule)

Details for Product anti-CD86 Antibody No. ABIN302084, Supplier: Log in to see
Antigen
  • B7-2
  • B7
  • B7.2
  • B70
  • CLS1
  • Cd28l2
  • ETC-1
  • Ly-58
  • Ly58
  • MB7
  • MB7-2
  • TS/A-2
  • CD28LG2
  • LAB72
  • CD86 molecule
  • CD86 antigen
  • CD86
  • Cd86
Alternatives
anti-Mouse (Murine) CD86 antibody for Flow Cytometry
Reactivity
Human
478
436
180
10
6
4
2
2
1
1
Host
Mouse
394
326
115
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD86 antibody is un-conjugated
99
98
57
52
21
17
16
14
10
10
9
9
8
6
5
5
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP), Western Blotting (WB)
641
266
179
177
135
106
90
69
54
54
42
14
13
12
11
10
8
4
2
2
2
1
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen B-lymphoblastoid cell line ARH 77
Clone BU63
Isotype IgG1
Specificity The antibody BU63 reacts with CD86 (B7-2), a 70 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
HLDA V, WS Code BP BP072
HLDA V, WS Code A A109
HLDA VI, WS Code BP 95
HLDA VI, WS Code B CD86.9
Cross-Reactivity (Details) Human, Other not determined
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Plasmids, Primers & others Plasmids, Primers & others CD86 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name CD86 (CD86 Antibody Abstract)
Background CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 Molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet, there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Flow Cytometry (FACS) image for anti-CD86 Molecule (CD86) antibody (ABIN302084) Surface staining of human peripheral blood cells with anti-CD86 (BU63) PerCP (monocyt...
Flow Cytometry (FACS) image for anti-CD86 Molecule (CD86) antibody (ABIN302084) Surface staining of human peripheral blood with anti-human CD44 (MEM-85) purified / G...
Product cited in: Hovden, Karlsen, Jonsson, Aarstad, Appel: "Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses." in: BMC immunology, Vol. 12, pp. 2, 2011 (PubMed).

Kolar, Mehta, Pelayo, Capra: "A novel human B cell subpopulation representing the initial germinal center population to express AID." in: Blood, Vol. 109, Issue 6, pp. 2545-52, 2007 (PubMed).

Chan, Baird, Mercer, Fleming: "Maturation and function of human dendritic cells are inhibited by orf virus-encoded interleukin-10." in: The Journal of general virology, Vol. 87, Issue Pt 11, pp. 3177-81, 2006 (PubMed).

Zhan, Towler, Calder: "The immunomodulatory role of human conjunctival epithelial cells." in: Investigative ophthalmology & visual science, Vol. 44, Issue 9, pp. 3906-10, 2003 (PubMed).

Mauri, Wyss-Coray, Gallati, Pichler: "Antigen-presenting T cells induce the development of cytotoxic CD4+ T cells. I. Involvement of the CD80-CD28 adhesion molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 1, pp. 118-27, 1995 (PubMed).

Caux, Vanbervliet, Massacrier, Azuma, Okumura, Lanier, Banchereau: "B70/B7-2 is identical to CD86 and is the major functional ligand for CD28 expressed on human dendritic cells." in: The Journal of experimental medicine, Vol. 180, Issue 5, pp. 1841-7, 1994 (PubMed).

Engel, Gribben, Freeman, Zhou, Nozawa, Abe, Nadler, Wakasa, Tedder: "The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen." in: Blood, Vol. 84, Issue 5, pp. 1402-7, 1994 (PubMed).

Background publications Yadav, Judkowski, Flodstrom-Tullberg, Sterling, Redmond, Sherman, Sarvetnick: "B7-2 (CD86) controls the priming of autoreactive CD4 T cell response against pancreatic islets." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 6, pp. 3631-9, 2004 (PubMed).

Vasilevko, Ghochikyan, Holterman, Agadjanyan: "CD80 (B7-1) and CD86 (B7-2) are functionally equivalent in the initiation and maintenance of CD4+ T-cell proliferation after activation with suboptimal doses of PHA." in: DNA and cell biology, Vol. 21, Issue 3, pp. 137-49, 2002 (PubMed).