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C-MYC antibody (Myc Proto-Oncogene protein) (C-Term) Primary Antibody

MYC Reactivity: Human FACS, IHC (p), IP, WB Host: Mouse Monoclonal 9E10 unconjugated
Pubmed (8)
Catalog No. ABIN302092
$400.75
Plus shipping costs $45.00
0.1 mg
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  • Target
    Binding Specificity
    C-Term
    Reactivity
    Human
    Host
    Mouse
    Clonality
    Monoclonal
    Conjugate
    This C-MYC antibody is un-conjugated
    Application
    Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
    Specificity
    The antibody 9E10 can be used to detect the c-Myc tag.
    Cross-Reactivity (Details)
    Human, Recognizes fusion proteins in all species
    Purification
    Purified by protein-A affinity chromatography
    Purity
    > 95 % (by SDS-PAGE)
    Immunogen
    Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of human c-Myc.
    Clone
    9E10
    Isotype
    IgG1
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
  • Jelínková, Šafaříková, Vondálová Blanářová, Skender, Hofmanová, Sova, Moyer, Kozubík, Kolář, Ehrmann, Hyršlová Vaculová: "Platinum(IV) complex LA-12 exerts higher ability than cisplatin to enhance TRAIL-induced cancer cell apoptosis via stimulation of mitochondrial pathway." in: Biochemical pharmacology, Vol. 92, Issue 3, pp. 415-24, 2014 (PubMed).

    Veracini, Simon, Richard, Schraven, Horejsi, Roche, Benistant: "The Csk-binding protein PAG regulates PDGF-induced Src mitogenic signaling via GM1." in: The Journal of cell biology, Vol. 182, Issue 3, pp. 603-14, 2008 (PubMed).

    Wang, Campoli, Ko, Luo, Ferrone: "Enhancement of scFv fragment reactivity with target antigens in binding assays following mixing with anti-tag monoclonal antibodies." in: Journal of immunological methods, Vol. 294, Issue 1-2, pp. 23-35, 2004 (PubMed).

    Fujiwara, Poikonen, Aleman, Valtavaara, Saksela, Mayer: "A single-chain antibody/epitope system for functional analysis of protein-protein interactions." in: Biochemistry, Vol. 41, Issue 42, pp. 12729-38, 2002 (PubMed).

    Baggio, Burgstaller, Hale, Putney, Lane, Lipovsek, Wright, Roberts, Liu, Szostak, Wagner: "Identification of epitope-like consensus motifs using mRNA display." in: Journal of molecular recognition : JMR, Vol. 15, Issue 3, pp. 126-34, 2002 (PubMed).

    Cermák, Símová, Pintzas, Horejsí, Andera: "Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules." in: The Journal of biological chemistry, Vol. 277, Issue 10, pp. 7955-61, 2002 (PubMed).

    Hilpert, Hansen, Wessner, Küttner, Welfle, Seifert, Höhne: "Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose." in: Protein engineering, Vol. 14, Issue 10, pp. 803-6, 2001 (PubMed).

    Siegel, Brandner, Hess: "Cross-reactivity of the monoclonal antibody 9E10 with murine c-MYC." in: International journal of oncology, Vol. 13, Issue 6, pp. 1259-62, 1999 (PubMed).

  • Target
    Alternative Name
    c-Myc (MYC Antibody Abstract)
    Synonyms
    MRTL, MYCC, bHLHe39, c-Myc, CMYC, c-myc, C-MYC, LOC100136746, RNCMYC, mMyc, AU016757, Myc2, Niard, Nird, MYC, cmyc, zc-myc, c-myc II, myc-B, myc2, CMyc, CG10798, D-Myc, DM, DMYc, Dm, Dmel\CG10798, Dmyc, EG:BACN5I9.1, anon-WO03040301.171, bHLHe57, c-MYC, d-myc, dMYC, dMyc, dMyc1, da, dm, dm/dMyc, dm/myc, dmyc, dmyc1, l(1)G0139, l(1)G0354, l(1)G0359, myc, MYC proto-oncogene, bHLH transcription factor, v-myc myelocytomatosis viral oncogene homolog, myelocytomatosis oncogene, MYC proto-oncogene, bHLH transcription factor a, MYC proto-oncogene, bHLH transcription factor L homeolog, v-myc myelocytomatosis viral oncogene homolog (avian), CG10798 gene product from transcript CG10798-RB, MYC, LOC100136746, Myc, myca, myc.L
    Background
    The c-myc gene (8q24 on human chromosome) is the cellular homologue of the v-myc gene originally isolated from an avian myelocytomatosis virus. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin-dependent kinase, ERK2 and C-Jun N-terminal Kinase (JNK) in cell proliferation and cell cycle regulation. In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated. c-Myc is also essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells.
    Pathways
    p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus
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